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All lanes : Anti-HIF1 alpha antibody (ab82832) at 1/1000 dilution
Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
Lane 2 : induced culture of E.coli (one shot Top10 competent cells)
Secondary
anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size : 93 kDa
Observed band size : 107 kDa (why is the actual band size different from the predicted?)
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
Molecular weight of tag is 14kDa.
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