Anti-HIF1 alpha antibody (ab82832)

helo my name is #### i need antibody for - hif1 alpha, hif2 alpha i need them to recognized human and mouse and i need it for westren blot if you can asist me because you have to mani option and i dont know what will be best   thank you    

ANSWER:

Thank you for your enquiry. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any HIF1 alpha or HIF2 alpha antibody tested in the species and application you are using should be suitable for your applications and species. Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet). I can confirm we have the following antibodies for HIF1 alpha tested in western blot, human and mouse. These would all be guaranteed for use in WB, hu and ms. ab1 Anti-HIF1 Mouse monoclonal alpha antibody [H1alpha67] - ChIP Grade Applications: ChIP, ICC/IF, IHC-Fr, IHC-P, IP, WB Reacts with: Cow, Ferret, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep  (3)    Click here (or use the following: http://www.abcam.com/index.html?datasheet=1).   ab113642 Mouse monoclonal Anti-HIF1 alpha antibody [1A3] Applications: ELISA, ICC/IF, IHC-P, WB Reacts with: Human, Monkey, Mouse  (7)  Click here (or use the following: http://www.abcam.com/index.html?datasheet=113642).        ab65979  Rabbit polyclonal Anti-HIF1 alpha antibody - Carboxyterminal end Applications: IHC-P, WB Reacts with: Human, Mouse, Rat    Click here (or use the following: http://www.abcam.com/index.html?datasheet=65979).        ab82832 Rabbit polyclonal Anti-HIF1 alpha antibody Applications:ELISA, WB Reacts with: Human, Mouse  Click here (or use the following: http://www.abcam.com/index.html?datasheet=82832). We have the following antibodies tested and guaranteed in Hu and WB for HIF2 alpha: ab109731 Rabbit polyclonal Anti-HIF2 alpha antibody Applications: ICC, ICC/IF, IHC-P, WB Reacts with: Human, Zebrafish Predicted Mouse, Rat, Cow, Pig   Click here (or use the following: http://www.abcam.com/index.html?datasheet=109731).          ab20654 Rabbit polyclonal Anti-HIF2 alpha antibody Applications: ICC/IF, IHC-P, WB Reacts with: Human, Rat Predicted: Mouse Click here (or use the following: http://www.abcam.com/index.html?datasheet=20654). I am sorry to confirm that as far as we are aware, non of our HIF2 alpha antibodies have been tested with samples from mouse. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.  For the antibodies I have quoted above, the alignment of the immunogen with the mouse protein is known to be high. It is therefore likely that these antibodies will detect in mouse, although we cannot guarantee this without testing.  If you would like to test one of the HIF2 alpha antibodies in mouse, please contact me again by replying to this message prior to the purchase as you may be eligible for our testing discount program. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: http://www.abcam.com/abreviews I look forward to hearing from you. Should you have any further questions please do not hesitate to ask.