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Western blot - HIF1 alpha antibody (ab82832)
(enlarge)
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Product Name
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HIF1 alpha antibody
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See all HIF1 alpha antibodies (24)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HIF1 alpha
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Immunogen
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3 synthetic peptides (human) conjugated to KLH
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
A minimum titer of 1/30000 is determined for one of the three peptides.
WB: 1/1000. Detects a band of approximately 107 kDa (predicted molecular weight: 93 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Induced culture of E.coli (one shot Top10 competent cells).
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Cellular localization
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Cytoplasmic in normoxia, nuclear translocation in response to hypoxia.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF
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Relevance
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Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1 alpha (HIF1 alpha) has been identified as a bHLH-PAS family member that is instrumental in the oxygen-dependent regulation of these genes. HIF1 alpha rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerises with the aryl hydrocarbon nuclear receptor translocator ARNT (also referred to as HIF1 beta). Heterodimerisation promotes efficient DNA binding.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum
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Purification notes
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Crude serum, final bleed.
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HIF1 alpha antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HIF1 alpha antibody images, references, products related to ab82832 and other tools.
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HIF1 alpha antibody images:
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 Western blot - HIF1 alpha antibody (ab82832)
All lanes : HIF1 alpha antibody (ab82832) at 1/1000 dilution
Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
Lane 2 : induced culture of E.coli (one shot Top10 competent cells)
Secondary
anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size : 93 kDa
Observed band size : 107 kDa (why is the actual band size different from the predicted?)
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
Molecular weight of tag is 14kDa.
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Search PubMed (MEDLINE) for references to HIF1 alpha
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HIF1 alpha antibody - more information
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Customer reviews (feedback) regarding HIF1 alpha antibody |
Customer FAQs regarding HIF1 alpha antibody |
Protocols for HIF1 alpha antibody |
Price and availability of products related to HIF1 alpha antibody |
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HIF1 alpha antibody - related products:
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HIF1 alpha antibody - other tools:
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Contact Abcam with a Technical Enquiry about HIF1 alpha antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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