HIF1 alpha antibody panel (ab10625)

Product Name 

HIF1 alpha antibody panel

Product type 

Kits

Description 

HIF1 alpha antibody panel

Immunogen 

Mab1 - ab463 = Fusion protein containing amino acids 432-528 of human HIF-1a.

Mab2 - ab8366 = Fusion protein containing amino acids 329-530 of human HIF-1a.

Pab1 - Fusion protein containing amino acids 530-825 of the mouse HIF1 alpha protein

Pab2 - Synthetic peptide corresponsing to the C-terminal of human HIF1 alpha protein

Specificity 

These antibodies are specific for HIF-1alpha.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF

Product overview 

This panel contains 50µl of the monoclonals and 25µl of the polyclonals of the following antibodies:

Mab1 - ab463 HIF1 alpha mouse monoclonal antibody; Clone H1alpha67; Isotype = IgG2b; Purity = Protein G; Concentration = 2.58mg/ml.

Mab2 - ab8366 HIF1 alpha mouse monoclonal antibody; Clone ESEE122; Isotype = IgG1; Purity = Protein G; Concentration = 2.2mg/ml.

Pab1 - HIF-1 alpha rabbit polyclonal antibody (amino acids 530-825); affinity purified;Concentration = 1.2mg/ml.

Pab2 - HIF-1 alpha rabbit polyclonal antibody (C-term);immunogen affinity purified;Concentration = 0.2mg/ml.

Storage instructions 

Store at +4°C.

Storage buffer 

Mab1 - ab463 HIF1 alpha mouse monoclonal antibody. Buffer = Cell culture supernatant; PBS; Sodium Azide.

Mab2 - ab8366 HIF1 alpha mouse monoclonal antibody. Buffer = Mouse Ascites; Sodium Azide.

Pab1 - 0.05% sodium azide

Pab2 - TBS with BSA;0.09% Sodium Azide

Form 

Liquid

Species Reactivity 

Mab1 - ab463 recognizes human, sheep, mouse, rat, porcine, rabbit and ferret HIF-1a. Bovine (-).

Mab2 - ab8366 recognizes human and rat. Mouse testing is in progress. Other species have not been tested.

Pab1 - reacts against mouse, rat, human and primate protein.
Other species have not been tested.

Pab2 - Reacts with human, monkey and mouse.
Other species have not been tested.

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100 - 1/500. A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background.
IHC-Fr: 1/100 - 1/500. A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background.(only ab8366 has been used on frozen sections). Antigen retrieval is strongly recommended.
WB: These antibodies work for Western blot analysis but are not specifically recommended for this application. Predicted molecular weight: 93 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Relevance 

Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1 alpha (HIF1 alpha) has been identified as a bHLH-PAS family member that is instrumental in the oxygen-dependent regulation of these genes. HIF1 alpha rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerises with the aryl hydrocarbon nuclear receptor translocator ARNT (also referred to as HIF1 beta). Heterodimerisation promotes efficient DNA binding.

Database links 

References for HIF1 alpha antibody panel (ab10625)

  This product has been used in: (two most recent references)

Chávez JC et al. Expression of hypoxia-inducible factor-1alpha in the brain of rats during chronic hypoxia. J Appl Physiol 89:1937-42 (2000). PubMed: 11053346

Zhong H et al. Overexpression of hypoxia-inducible factor 1alpha in common human cancers and their metastases. Cancer Res 59:5830-5 (1999). PubMed: 10582706
If you publish research using ab10625 please let us know so that we can cite the reference on this datasheet.

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