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Products:Cardiovascular >> Hypoxia >> HIF

HIF1 alpha antibody panel (ab10625)

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Overview

Product name

HIF1 alpha antibody panel

Specificity

These antibodies are specific for HIF-1alpha.

Cross reactivity

Reacts with

Mouse, Rat, Sheep, Rabbit, Human, Pig, Ferret

Product overview

This panel contains 50µl of the monoclonals and 25µl of the polyclonals of the following antibodies:

Mab1 - ab463 HIF1 alpha mouse monoclonal antibody; Clone H1alpha67; Isotype = IgG2b; Purity = Protein G; Concentration = 2.0mg/ml.

Mab2 - ab8366 HIF1 alpha mouse monoclonal antibody; Clone ESEE122; Isotype = IgG1; Purity = Protein G; Concentration = 1.0mg/ml.

Pab1 - HIF-1 alpha rabbit polyclonal antibody (amino acids 530-825); affinity purified;Concentration = 1.0mg/ml.

Pab2 - HIF-1 alpha rabbit polyclonal antibody (C-term);immunogen affinity purified;Concentration = 0.2mg/ml.

Tested applications

IHC-P, IHC-Fr, WBmore details

Properties

Storage instructions

Store at +4°C.

Storage buffer

Mab1 - ab463 HIF1 alpha mouse monoclonal antibody. Buffer = Cell culture supernatant; PBS; Sodium Azide.

Mab2 - ab8366 HIF1 alpha mouse monoclonal antibody. Buffer = Mouse Ascites; Sodium Azide.

Pab1 - 0.05% sodium azide

Pab2 - TBS with BSA;0.09% Sodium Azide

Components 1 kit
ab106251 unit
Mab1 - ab463 HIF1 alpha mouse monoclonal antibody; Clone H1alpha67; Isotype = IgG2b; .NB100-1231 x 50µl
Mab2 - ab8366 HIF1 alpha mouse monoclonal antibody; Clone ESEE122; Isotype = IgG1;.NB100-1311 x 50µl
Pab1 - HIF-1 alpha rabbit polyclonal antibody (amino acids 530-825);.NB100-4791 x 25µl
Pab2 - HIF-1 alpha rabbit polyclonal antibody (C-term);.NB100-4491 x 25µl

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF

Function

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.

Tissue specificity

Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.

Domain

Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).

Post-translational
modifications

In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
Requires phosphorylation for DNA-binding.
Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

Cellular localization

Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.

Target information above from: UniProt accessionQ16665 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Basic helix loop helix PAS protein MOP1
  • ARNT interacting protein
  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • bHLHe78
  • Class E basic helix-loop-helix protein 78
  • HIF 1A
  • HIF 1alpha
  • HIF-1-alpha
  • HIF1 A
  • HIF1 Alpha
  • HIF1-alpha
  • HIF1A
  • HIF1A_HUMAN
  • Hypoxia inducible factor 1 alpha
  • Hypoxia inducible factor 1 alpha isoform I.3
  • Hypoxia inducible factor 1 alpha subunit
  • Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor
  • Hypoxia-inducible factor 1-alpha
  • Member of PAS protein 1
  • Member of PAS superfamily 1
  • Member of the PAS Superfamily 1
  • MOP 1
  • MOP1
  • PAS domain-containing protein 8
  • PASD 8
  • PASD8
see all

Database links

see all

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    IHC-P

     IHC-P: 1/100 - 1/500.(A ...Read more →

    IHC-P: 1/100 - 1/500.(A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background.)

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    IHC-Fr

      See more...Read more →

    IHC-Fr: 1/100 - 1/500.(A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background.(only ab8366 has been used on frozen sections). Antigen retrieval is strongly recommended.)

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    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Predicted molecular weight: 93 kDa.(These antibodies work for Western blot analysis but are not specifically recommended for this application.)

Protocols

  • WB protocol using MEF cells- Hif1 alpha
  • Nuclear Extract Preparation for HIF 1 alpha

    • References for HIF1 alpha antibody panel (ab10625)

    This product has been referenced in:

    • Chávez JCet al. Expression of hypoxia-inducible factor-1alpha in the brain of rats during chronic hypoxia. J Appl Physiol 89:1937-42 (2000).Read more (PubMed: 11053346) »
    • Zhong Het al. Overexpression of hypoxia-inducible factor 1alpha in common human cancers and their metastases. Cancer Res 59:5830-5 (1999).Read more (PubMed: 10582706) »

    See all 2 publications for this product

    Publishing research using ab10625? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"