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ab2771 has been referenced in 5 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-HIF1 beta antibody [2B10] (ab2771) at 1/2000 dilution + Human U2OS cells - whole cell lysate at 20 µg
Secondary
HRP conjugated goat anti-mouse antibody
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 86 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible non-specific binding).
Exposure time : 5 minutes
This image is courtesy of an anonymous Abreview
IHC image of ab2771 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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