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Products:Cardiovascular >> Hypoxia >> HIF

Anti-HIF1 beta antibody [2B10] (ab2771)

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Overview

Product name

Anti-HIF1 beta antibody [2B10]
See all HIF1 beta products (11) ...

Description

Mouse monoclonal [2B10] to HIF1 beta

Specificity

Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).

Tested applications

IF, IHC-P, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human, African Green Monkey

Immunogen

Synthetic peptide, corresponding to amino acids 771-789 of Human HIF1 beta.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS, 1 mg/ml BSA, 0.05% sodium azide

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

2B10

Isotype

IgG1

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF

  • Western blot - HIF1 beta antibody [2B10] (ab2771)Western blot - HIF1 beta antibody [2B10] (ab2771) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-HIF1 beta antibody [2B10](ab2771)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-HIF1 beta antibody [2B10](ab2771) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP27540 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • nuclear translocator antibody
  • ARNT antibody
  • ARNT protein antibody
  • ARNT_HUMAN antibody
  • Aryl Hydrocarbon Receptor Nuclear Translocator antibody
  • bHLHe2 antibody
  • Class E basic helix-loop-helix protein 2 antibody
  • Dioxin Receptor antibody
  • Dioxin receptor nuclear translocator antibody
  • Drnt antibody
  • HIF 1 beta antibody
  • HIF 1beta antibody
  • HIF-1-beta antibody
  • HIF1-beta antibody
  • HIF1B antibody
  • HIF1beta antibody
  • Hypoxia Inducible Factor 1 antibody
  • Hypoxia inducible factor 1 beta antibody
  • Hypoxia-inducible factor 1-beta antibody
  • Tango antibody
see all

Database links

see all

Anti-HIF1 beta antibody [2B10] images:

  Western blot - HIF1 beta antibody [2B10] (ab2771)

Western blot - HIF1 beta antibody [2B10] (ab2771)

Anti-HIF1 beta antibody [2B10] (ab2771) at 1/2000 dilution + Human U2OS cells - whole cell lysate at 20 µg

Secondary
HRP conjugated goat anti-mouse antibody
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 86 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible non-specific binding).

Exposure time : 5 minutes

This image is courtesy of an anonymous Abreview

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-HIF1 beta antibody [2B10](ab2771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-HIF1 beta antibody [2B10](ab2771)

IHC image of ab2771 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-HIF1 beta antibody [2B10] (ab2771)

This product has been referenced in:

  • Liu S & Piatigorsky J Regulation of Mouse Small Heat Shock Protein ab-Crystallin Gene by Aryl Hydrocarbon Receptor. PLoS One 6:e17904 (2011). WB; Mouse.Read more (PubMed: 21494593) »
  • Lee SJet al. Beclin 1 deficiency is associated with increased hypoxia-induced angiogenesis. Autophagy 7:829-39 (2011).Read more (PubMed: 21685724) »

See all 5 publications for this product

Publishing research using ab2771? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"