HIF1 beta antibody [2B10] (ab2771)

Product Name 

HIF1 beta antibody [2B10]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2B10] to HIF1 beta

Immunogen 

Synthetic peptide, corresponding to amino acids 771-789 of Human HIF1 beta.

Reacts with 
(species key)

Hu, Ms, Rat, NHuPrm

Specificity 

Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IF: 1/1000. Staining of ARNT in VT{2} cells (Hepa 1-C4T mutant deficient in ARNT function but stably transfected with the full length human ARNT cDNA) using ab2771 yields non-nucleolar, nuclear staining only.
IP: 1-2ug/IP
WB: 1/4000. Detects a band of approximately 87 kDa (predicted molecular weight: 86 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF

Relevance 

HIF1 beta (hypoxia-inducible factor 1 beta, or aryl hydrocarbon receptor nuclear transporter, ARNT) is a transcription factor that contains a basic Helix-Loop-Helix (bHLH) motif as well as two PAS domains. HIF1 beta is required for the activity of the aryl hydrocarbon (Ah) receptor, which is involved in the induction of several enzymes that are involved in xenobiotic metabolism. Binding of ligands such as dioxin to the Ah receptor result in the translocation of the Ah receptor from the cytosol to the nucleus through its association with HIF1 beta. This complex then initiates transcription of genes involved in the activation of PAH procarcinogens. Efficient DNA binding of HIF1 beta requires heterodimerisation with another bHLH protein, such as the Ah receptor.

HIF1 beta also plays a critical role in the transcriptional regulation of the adaptive response to hypoxia by forming heterodimers with HIF1 alpha and EPAS1/HIF2 alpha. Hypoxia-induced gene transcription contributes substantially to the malignant phenotype of solid tumours by promoting characteristic tumour behaviour such as angiogenesis, invasion, metastasis, de-differentiation and enhanced glycolytic metabolism.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2B10

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

PBS, 1 mg/ml BSA, 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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HIF1 beta antibody [2B10] images:

References for HIF1 beta antibody [2B10] (ab2771)

  This product has been used in:

Hord NG & Perdew GH Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear translocator. Mol Pharmacol 46:618-26 (1994). PubMed: 7526149
If you publish research using ab2771 please let us know so that we can cite the reference on this datasheet.

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