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Overview

  • Product nameAnti-HIF1 beta antibody [3D10]See all HIF1 beta primary antibodies ...
  • Description
    Mouse monoclonal [3D10] to HIF1 beta
  • Tested applicationsWB, IHC-P, ICC/IF, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to N terminal amino acids 1-110 of Human HIF1 beta, with a 26 kDa proprietary tag (AAH60838).

  • Positive controlHuman testis tissue; HIF1 beta-transfected 293T cell lysate; HeLa nuclear extract; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab124359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 87 kDa.
IHC-P IHC-P: Use a concentration of 5 µg/ml.
ICC/IF ICC/IF: Use a concentration of 10 µg/ml.
Sandwich ELISA sELISA: Use at an assay dependent concentration.

Target

  • FunctionRequired for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P27540 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        arnT antibodyARNT protein antibodyARNT_HUMAN antibody
        Aryl hydrocarbon receptor nuclear translocator antibodybHLHe2 antibodyClass E basic helix-loop-helix protein 2 antibodyDioxin receptor antibodyDioxin receptor nuclear translocator antibodyDrnt antibodyHIF 1 beta antibodyHIF 1beta antibodyHIF-1-beta antibodyHIF1-beta antibodyHIF1B antibodyHIF1beta antibodyHypoxia Inducible Factor 1 antibodyHypoxia inducible factor 1 beta antibodyHypoxia-inducible factor 1-beta antibodynuclear translocator antibodyTango antibody
      see all

    Anti-HIF1 beta antibody [3D10] images

    • Western blot - Anti-HIF1 beta antibody [3D10] (ab124359)
      Western blot - Anti-HIF1 beta antibody [3D10] (ab124359)
      Anti-HIF1 beta antibody [3D10] (ab124359) at 1/500 dilution + HeLa nuclear extract

      Predicted band size : 87 kDa
    • Western blot - Anti-HIF1 beta antibody [3D10] (ab124359)
      Western blot - Anti-HIF1 beta antibody [3D10] (ab124359)
      All lanes : Anti-HIF1 beta antibody [3D10] (ab124359) at 1/500 dilution

      Lane 1 : 293T cell lysate, transfected with HIF1 beta
      Lane 2 : 293T cell lysate, non transfected


      Predicted band size : 87 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [3D10] (ab124359)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [3D10] (ab124359)
      ab124359, at 5 µg/ml, staining HIF1 beta in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [3D10] (ab124359)
      Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [3D10] (ab124359)
      ab124359, at 10 µg/ml, staining HIF1 beta in HeLa cells by Immunofluorescence.
    • Sandwich ELISA - Anti-HIF1 beta antibody [3D10] (ab124359)
      Sandwich ELISA - Anti-HIF1 beta antibody [3D10] (ab124359)
      Using ab124359 as a capture antibody, the detection limit for recombinant proprietary tagged HIF1 beta is approximately 0.03ng/ml.

    References for Anti-HIF1 beta antibody [3D10] (ab124359)

    ab124359 has not yet been referenced specifically in any publications.

    Publishing research using ab124359 ? Please let us know so that we can cite the reference in this datasheet

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