Overview

  • Product nameAnti-HIF1 beta antibody [3D10]See all HIF1 beta primary antibodies ...
  • Description
    Mouse monoclonal [3D10] to HIF1 beta
  • Tested applicationsWB, IHC-P, ICC/IF, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to N terminal amino acids 1-110 of Human HIF1 beta, with a 26 kDa proprietary tag (AAH60838).

  • Positive control
    • Human testis tissue; HIF1 beta-transfected 293T cell lysate; HeLa nuclear extract; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab124359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 87 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

Target

  • FunctionRequired for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • arnT antibody
    • ARNT protein antibody
    • ARNT_HUMAN antibody
    • Aryl hydrocarbon receptor nuclear translocator antibody
    • bHLHe2 antibody
    • Class E basic helix-loop-helix protein 2 antibody
    • Dioxin receptor antibody
    • Dioxin receptor nuclear translocator antibody
    • Drnt antibody
    • HIF 1 beta antibody
    • HIF 1beta antibody
    • HIF-1-beta antibody
    • HIF1-beta antibody
    • HIF1B antibody
    • HIF1beta antibody
    • Hypoxia Inducible Factor 1 antibody
    • Hypoxia inducible factor 1 beta antibody
    • Hypoxia-inducible factor 1-beta antibody
    • nuclear translocator antibody
    • Tango antibody
    see all

Anti-HIF1 beta antibody [3D10] images

  • Anti-HIF1 beta antibody [3D10] (ab124359) at 1/500 dilution + HeLa nuclear extract

    Predicted band size : 87 kDa
  • All lanes : Anti-HIF1 beta antibody [3D10] (ab124359) at 1/500 dilution

    Lane 1 : 293T cell lysate, transfected with HIF1 beta
    Lane 2 : 293T cell lysate, non transfected


    Predicted band size : 87 kDa
  • ab124359, at 5 µg/ml, staining HIF1 beta in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry.
  • ab124359, at 10 µg/ml, staining HIF1 beta in HeLa cells by Immunofluorescence.
  • Using ab124359 as a capture antibody, the detection limit for recombinant proprietary tagged HIF1 beta is approximately 0.03ng/ml.

References for Anti-HIF1 beta antibody [3D10] (ab124359)

ab124359 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"