HIF1 beta antibody - ChIP Grade (ab2)

Product Name 

HIF1 beta antibody - ChIP Grade

Product type 

Primary antibodies

Description 

Rabbit polyclonal to HIF1 beta - ChIP Grade

Immunogen 

Fusion protein, containing amino acids 496-789 of Human HIF-1 beta.

Reacts with 
(species key)

Hu, Ms, Rat, Cow, Frt, Shp

Specificity 

This antibody is specific for HIF-1 beta/ARNT. It is not known if ab2 crossreacts with ARNT2 (which is related but a different gene product).

Tested applications 
(see key)

ChIP, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ChIP: Use 0.2µg for µg of chromatin
IP: Use at an assay dependent dilution.
WB: 1/2000. Predicted molecular weight: 92 kDa.
Background non-specific bands are minimal on MCF7 human nuclear extracts. Background may increase with whole cell extract preparations.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MCF7 human nuclear extracts

Cellular localization 

Nuclear

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF

Relevance 

HIF1 beta (hypoxia-inducible factor 1 beta, or aryl hydrocarbon receptor nuclear transporter, ARNT) is a transcription factor that contains a basic Helix-Loop-Helix (bHLH) motif as well as two PAS domains. HIF1 beta is required for the activity of the aryl hydrocarbon (Ah) receptor, which is involved in the induction of several enzymes that are involved in xenobiotic metabolism. Binding of ligands such as dioxin to the Ah receptor result in the translocation of the Ah receptor from the cytosol to the nucleus through its association with HIF1 beta. This complex then initiates transcription of genes involved in the activation of PAH procarcinogens. Efficient DNA binding of HIF1 beta requires heterodimerisation with another bHLH protein, such as the Ah receptor.

HIF1 beta also plays a critical role in the transcriptional regulation of the adaptive response to hypoxia by forming heterodimers with HIF1 alpha and EPAS1/HIF2 alpha. Hypoxia-induced gene transcription contributes substantially to the malignant phenotype of solid tumours by promoting characteristic tumour behaviour such as angiogenesis, invasion, metastasis, de-differentiation and enhanced glycolytic metabolism.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

serum with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for HIF1 beta antibody - ChIP Grade images, references, products related to ab2 and other tools.

HIF1 beta antibody - ChIP Grade images:

References for HIF1 beta antibody - ChIP Grade (ab2)

  This product has been used in:

Echevarr a M et al. Development of cytosolic hypoxia and hypoxia-inducible factor stabilization are facilitated by aquaporin-1 expression. J Biol Chem 282:30207-15 (2007). WB; Rat. PubMed: 17673462



If you publish research using ab2 please let us know so that we can cite the reference on this datasheet.

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