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Products:Cardiovascular >> Hypoxia >> HIF
Overview
Product name
Anti-HIF1 beta antibody [H1beta234]
See all HIF1 beta products (11) ...
Description
Mouse monoclonal [H1beta234] to HIF1 beta
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Cow, Human, Ferret
Immunogen
Fusion protein containing amino acids 496-789 (Human).
Epitope
496-789 of human HIF-1beta
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
H1beta234
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IHC-P
Application notes
IHC-P: 1/1000. Incubate overnight at 4 C
WB: 1/1500. Predicted molecular weight: 92 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP27540
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- nuclear translocator antibody
- ARNT antibody
- ARNT protein antibody
see all
Database links
- Entrez Gene: 281010 Cow
- Entrez Gene: 405 Human
- Entrez Gene: 11863 Mouse
- Entrez Gene: 25242 Rat
- Omim: 126110 Human
- SwissProt: Q9BE97 Cow
- SwissProt: P27540 Human
- SwissProt: P53762 Mouse
see all
References for Anti-HIF1 beta antibody [H1beta234] (ab465)
This product has been referenced in:
- Critchley HOet al. Hypoxia-inducible factor-1alpha expression in human endometrium and its regulation by prostaglandin E-series prostanoid receptor 2 (EP2). Endocrinology 147:744-53 (2006).Read more (PubMed: 16282352) »
- Mabjeesh NJet al. Geldanamycin induces degradation of hypoxia-inducible factor 1alpha protein via the proteosome pathway in prostate cancer cells. Cancer Res 62:2478-82 (2002).Read more (PubMed: 11980636) »
See all 6 publications for this product
Publishing research using ab465? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"