|
|
|
|
|
Product Name
|
HIF1 beta antibody
|
| |
See all HIF1 beta antibodies (8)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to HIF1 beta
|
|
Immunogen
|
Synthetic peptide: SYNNEEFPDLTMFPPFSE, corresponding to amino acids 771-789 of Human HIF-1beta. This sequence is completely conserved between human and mouse ARNT.
BLAST 'SYNNEEFPDLTMFPPFSE' with  or 
|
|
Reacts with
|
Ms
|
|
Predicted to react
(species key)
|
Hu, Rat, Cow
(Due to sequence homology)
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: 1/500. Detects a band of approximately 87 kDa (predicted molecular weight: 94 kDa).
ab5624 reacts with one other band lower than ARNT whose identity has not been established.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Hepa 1c1c7 cell lysate.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF
|
|
Relevance
|
HIF1 beta (hypoxia-inducible factor 1 beta, or aryl hydrocarbon receptor nuclear transporter, ARNT) is a transcription factor that contains a basic Helix-Loop-Helix (bHLH) motif as well as two PAS domains. HIF1 beta is required for the activity of the aryl hydrocarbon (Ah) receptor, which is involved in the induction of several enzymes that are involved in xenobiotic metabolism. Binding of ligands such as dioxin to the Ah receptor result in the translocation of the Ah receptor from the cytosol to the nucleus through its association with HIF1 beta. This complex then initiates transcription of genes involved in the activation of PAH procarcinogens. Efficient DNA binding of HIF1 beta requires heterodimerisation with another bHLH protein, such as the Ah receptor.
HIF1 beta also plays a critical role in the transcriptional regulation of the adaptive response to hypoxia by forming heterodimers with HIF1 alpha and EPAS1/HIF2 alpha. Hypoxia-induced gene transcription contributes substantially to the malignant phenotype of solid tumours by promoting characteristic tumour behaviour such as angiogenesis, invasion, metastasis, de-differentiation and enhanced glycolytic metabolism.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.600 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HIF1 beta antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to HIF1 beta
|
| |
HIF1 beta antibody - more information
|
Customer reviews (feedback) regarding HIF1 beta antibody |
Customer FAQs regarding HIF1 beta antibody |
Protocols for HIF1 beta antibody |
Price and availability of products related to HIF1 beta antibody |
| |
HIF1 beta antibody - related products:
|
|
|
| |
HIF1 beta antibody - other tools:
|
Contact Abcam with a Technical Enquiry about HIF1 beta antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
