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Products:Cardiovascular >> Hypoxia >> HIF
Overview
Product name
Anti-HIF1 beta antibody
See all HIF1 beta products (11) ...
Description
Rabbit polyclonal to HIF1 beta
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Chicken, Cow, Human
Immunogen
Synthetic peptide: SYNNEEFPDLTMFPPFSE, corresponding to amino acids 771-789 of Human HIF-1beta. This sequence is completely conserved between human and mouse ARNT.
SYNNEEFPDL TMFPPFSE
Positive control
Hepa 1c1c7 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/500. Detects a band of approximately 87 kDa (predicted molecular weight: 94 kDa).
ab5624 reacts with one other band lower than ARNT whose identity has not been established.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP27540
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- nuclear translocator antibody
- ARNT antibody
- ARNT protein antibody
see all
Database links
- Entrez Gene: 374026 Chicken
- Entrez Gene: 281010 Cow
- Entrez Gene: 405 Human
- Entrez Gene: 11863 Mouse
- Entrez Gene: 25242 Rat
- Omim: 126110 Human
- SwissProt: Q9BE97 Cow
- SwissProt: P27540 Human
see all
References for Anti-HIF1 beta antibody (ab5624)
ab5624 has not yet been referenced specifically in any publications.
Publishing research using ab5624? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"