Anti-HIF1AN antibody (ab12289)
- Product nameAnti-HIF1AN antibodySee all HIF1AN primary antibodies ...
- DescriptionRabbit polyclonal to HIF1AN
- SpecificityThe expected molecular weight of FIH is 40 kDa. A band corresponding to this size is seen on the western blot analysis, and is competed away by the addition of FIH blocking peptide. A smaller band is seen, which is also competed away by the blocking peptide. In the A431 extract a nonspecific band of approximately 50 kDa is seen, which is not diminished by the addition of blocking peptide.
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Human
Synthetic peptide derived from within residues 250 to the C-terminus of Human FIH/asparagine hydroxylase.
- Positive controlThis antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293 and Hela Nuclear lysate.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab12289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Can be blocked with FIH peptide (ab12306).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
- Sequence similaritiesContains 1 JmjC domain.
- Cellular localizationNucleus.
- DKFZp762F1811 antibodyFactor inhibiting HIF 1 antibodyFactor inhibiting HIF-1 antibody
- Factor inhibiting HIF1 antibodyFIH 1 antibodyFIH-1 antibodyFIH1 antibodyFLJ20615 antibodyFLJ22027 antibodyHIF1AN antibodyHIF1N_HUMAN antibodyHypoxia inducible factor 1 alpha inhibitor antibodyHypoxia inducible factor 1 alpha subunit inhibitor antibodyHypoxia inducible factor asparagine hydroxylase antibodyHypoxia-inducible factor 1-alpha inhibitor antibodyHypoxia-inducible factor asparagine hydroxylase antibodyPeptide aspartate beta dioxygenase antibodyprolyl hydroxylase antibody
Anti-HIF1AN antibody images
All lanes : Anti-HIF1AN antibody (ab12289) at 1/500 dilution
Lane 1 : Hela nuclear extract
Lane 2 : Hela whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : Hek293 cell lysate
Lane 6 : Hela nuclear extract with FIH blocking peptide at 1 µg/ml
Lane 7 : Hela whole cell lysate
Lane 8 : A431 whole cell lysate with FIH blocking peptide at 1 µg/ml
Lane 9 : Jurkat whole cell lysate with FIH blocking peptide at 1 µg/ml
Lane 10 : Hek293 whole cell lysate with FIH blocking peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Goat polyclonal to rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size : 40 kDa
Observed band size : 40 kDa
Exposure time : 5 minutes
ICC/IF image of ab12289 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab12289, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-HIF1AN antibody (ab12289)
This product has been referenced in:
- Zolk O et al. Activation of negative regulators of the hypoxia-inducible factor (HIF) pathway in human end-stage heart failure. Biochem Biophys Res Commun 376:315-20 (2008). WB ; Human . Read more (PubMed: 18782560) »