Anti-HIF1AN antibody (ab12289)

Overview

• Product nameAnti-HIF1AN antibodySee all HIF1AN primary antibodies ...
• Description
  Rabbit polyclonal to HIF1AN
• SpecificityThe expected molecular weight of FIH is 40 kDa. A band corresponding to this size is seen on the western blot analysis, and is competed away by the addition of FIH blocking peptide. A smaller band is seen, which is also competed away by the blocking peptide. In the A431 extract a nonspecific band of approximately 50 kDa is seen, which is not diminished by the addition of blocking peptide.
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide derived from within residues 250 to the C-terminus of Human FIH/asparagine hydroxylase.

  Read Abcam's proprietary immunogen policy

  .

• Positive controlThis antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293 and Hela Nuclear lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • Hypoxia
  • HIF

  • Cancer
  • Invasion/microenvironment
  • Hypoxia
  • Hydroxylases

  • Cardiovascular
  • Hypoxia
  • HIF

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Methylation

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Jumonji containing proteins

  • Epigenetics and Nuclear Signaling
  • Cardiovascular/Immune
  • Hypoxia
  • HIF

Applications

Target

• FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
• Sequence similaritiesContains 1 JmjC domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9NWT6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Clone ID: BC007719 Human
  • Entrez Gene: 55662 Human
  • IMAGE Id: 4138066 Human
  • Omim: 606615 Human
  • SwissProt: Q5W147 Human
  • SwissProt: Q9NWT6 Human
  • Unigene: 500788 Human

• Alternative names
  DKFZp762F1811 antibodyFactor inhibiting HIF 1 antibodyFactor inhibiting HIF-1 antibody

  Factor inhibiting HIF1 antibodyFIH 1 antibodyFIH-1 antibodyFIH1 antibodyFLJ20615 antibodyFLJ22027 antibodyHIF1AN antibodyHIF1N_HUMAN antibodyHypoxia inducible factor 1 alpha inhibitor antibodyHypoxia inducible factor 1 alpha subunit inhibitor antibodyHypoxia inducible factor asparagine hydroxylase antibodyHypoxia-inducible factor 1-alpha inhibitor antibodyHypoxia-inducible factor asparagine hydroxylase antibodyPeptide aspartate beta dioxygenase antibodyprolyl hydroxylase antibody

  see all

Anti-HIF1AN antibody images

• 

  Western blot - HIF1AN antibody (ab12289)
  All lanes : Anti-HIF1AN antibody (ab12289) at 1/500 dilution
  
  Lane 1 : Hela nuclear extract
  Lane 2 : Hela whole cell lysate
  Lane 3 : A431 whole cell lysate
  Lane 4 : Jurkat whole cell lysate
  Lane 5 : Hek293 cell lysate
  Lane 6 : Hela nuclear extract with FIH blocking peptide at 1 µg/ml
  Lane 7 : Hela whole cell lysate
  Lane 8 : A431 whole cell lysate with FIH blocking peptide at 1 µg/ml
  Lane 9 : Jurkat whole cell lysate with FIH blocking peptide at 1 µg/ml
  Lane 10 : Hek293 whole cell lysate with FIH blocking peptide at 1 µg/ml
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal to rabbit IgG H&L (HRP) at 1/5000 dilution
  
  Predicted band size : 40 kDa
  Observed band size : 40 kDa
  
  
  Exposure time : 5 minutes
• 

  Immunocytochemistry/ Immunofluorescence - FIH/asparagine hydroxylase antibody (ab12289)
  ICC/IF image of ab12289 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab12289, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).