Overview

  • Product name
  • Description
    Rabbit polyclonal to HIF1AN
  • Specificity
    The expected molecular weight of FIH is 40 kDa. A band corresponding to this size is seen on the western blot analysis, and is competed away by the addition of FIH blocking peptide. A smaller band is seen, which is also competed away by the blocking peptide. In the A431 extract a nonspecific band of approximately 50 kDa is seen, which is not diminished by the addition of blocking peptide.
  • Tested applications
    Suitable for: IHC-FoFr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Chicken, Human
  • Immunogen

    Synthetic peptide derived from within residues 250 to the C-terminus of Human FIH/asparagine hydroxylase.

    .

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293 and Hela Nuclear lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab12289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
ICC/IF
WB
  • Application notes
    ICC/IF: Use at a concentration of 5 µg/ml.
    WB: 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Can be blocked with FIH peptide (ab12306).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
    • Sequence similarities
      Contains 1 JmjC domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp762F1811 antibody
      • Factor inhibiting HIF-1 antibody
      • Factor inhibiting HIF1 antibody
      • FIH 1 antibody
      • FIH-1 antibody
      • FIH1 antibody
      • FLJ20615 antibody
      • FLJ22027 antibody
      • HIF1AN antibody
      • HIF1N_HUMAN antibody
      • Hypoxia inducible factor 1 alpha inhibitor antibody
      • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
      • Hypoxia inducible factor asparagine hydroxylase antibody
      • Hypoxia-inducible factor 1-alpha inhibitor antibody
      • Hypoxia-inducible factor asparagine hydroxylase antibody
      • Peptide aspartate beta dioxygenase antibody
      see all

    Images

    • All lanes : Anti-HIF1AN antibody (ab12289) at 1/500 dilution

      Lane 1 : Hela nuclear extract
      Lane 2 : Hela whole cell lysate
      Lane 3 : A431 whole cell lysate
      Lane 4 : Jurkat whole cell lysate
      Lane 5 : Hek293 cell lysate
      Lane 6 : Hela nuclear extract with FIH blocking peptide at 1 µg/ml
      Lane 7 : Hela whole cell lysate
      Lane 8 : A431 whole cell lysate with FIH blocking peptide at 1 µg/ml
      Lane 9 : Jurkat whole cell lysate with FIH blocking peptide at 1 µg/ml
      Lane 10 : Hek293 whole cell lysate with FIH blocking peptide at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to rabbit IgG H&L (HRP) at 1/5000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40 kDa


      Exposure time : 5 minutes
    • ICC/IF image of ab12289 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab12289, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    References

    This product has been referenced in:
    • Yang Y  et al. A novel fumarate hydratase-deficient HLRCC kidney cancer cell line, UOK268: a model of the Warburg effect in cancer. Cancer Genet 205:377-90 (2012). Read more (PubMed: 22867999) »
    • Zolk O  et al. Activation of negative regulators of the hypoxia-inducible factor (HIF) pathway in human end-stage heart failure. Biochem Biophys Res Commun 376:315-20 (2008). WB ; Human . Read more (PubMed: 18782560) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Thanks for your call yesterday and for your patience. We have two HIF1AN antibodies that have been tested with mouse samples and are guaranteed to work in mouse: ab92304 and ab63163- http://www.abcam.com/HIF1AN-antibody-ab63163.html ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293, Jurkat, Raw264)
    Loading amount
    20 µg
    Specification
    HEK293, Jurkat, Raw264
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Feb 24 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa and SK-N-SH)
    Specification
    HeLa and SK-N-SH
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jul 24 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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