Overview

  • Product nameAnti-HIF1AN antibodySee all HIF1AN primary antibodies ...
  • Description
    Rabbit polyclonal to HIF1AN
  • SpecificityThe expected molecular weight of FIH is 40 kDa. A band corresponding to this size is seen on the western blot analysis, and is competed away by the addition of FIH blocking peptide. A smaller band is seen, which is also competed away by the blocking peptide. In the A431 extract a nonspecific band of approximately 50 kDa is seen, which is not diminished by the addition of blocking peptide.
  • Tested applicationsIHC-FoFr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Chicken, Human
  • Immunogen

    Synthetic peptide derived from within residues 250 to the C-terminus of Human FIH/asparagine hydroxylase.

    .

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293 and Hela Nuclear lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab12289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
ICC/IF
WB
  • Application notesICC/IF: Use at a concentration of 5 µg/ml.
    WB: 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Can be blocked with FIH peptide (ab12306).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
    • Sequence similaritiesContains 1 JmjC domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp762F1811 antibody
      • Factor inhibiting HIF 1 antibody
      • Factor inhibiting HIF-1 antibody
      • Factor inhibiting HIF1 antibody
      • FIH 1 antibody
      • FIH-1 antibody
      • FIH1 antibody
      • FLJ20615 antibody
      • FLJ22027 antibody
      • HIF1AN antibody
      • HIF1N_HUMAN antibody
      • Hypoxia inducible factor 1 alpha inhibitor antibody
      • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
      • Hypoxia inducible factor asparagine hydroxylase antibody
      • Hypoxia-inducible factor 1-alpha inhibitor antibody
      • Hypoxia-inducible factor asparagine hydroxylase antibody
      • Peptide aspartate beta dioxygenase antibody
      • prolyl hydroxylase antibody
      see all

    Anti-HIF1AN antibody images

    • All lanes : Anti-HIF1AN antibody (ab12289) at 1/500 dilution

      Lane 1 : Hela nuclear extract
      Lane 2 : Hela whole cell lysate
      Lane 3 : A431 whole cell lysate
      Lane 4 : Jurkat whole cell lysate
      Lane 5 : Hek293 cell lysate
      Lane 6 : Hela nuclear extract with FIH blocking peptide at 1 µg/ml
      Lane 7 : Hela whole cell lysate
      Lane 8 : A431 whole cell lysate with FIH blocking peptide at 1 µg/ml
      Lane 9 : Jurkat whole cell lysate with FIH blocking peptide at 1 µg/ml
      Lane 10 : Hek293 whole cell lysate with FIH blocking peptide at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to rabbit IgG H&L (HRP) at 1/5000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40 kDa


      Exposure time : 5 minutes
    • ICC/IF image of ab12289 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab12289, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    References for Anti-HIF1AN antibody (ab12289)

    This product has been referenced in:
    • Zolk O  et al. Activation of negative regulators of the hypoxia-inducible factor (HIF) pathway in human end-stage heart failure. Biochem Biophys Res Commun 376:315-20 (2008). WB ; Human . Read more (PubMed: 18782560) »
    • Zheng X  et al. Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways. Proc Natl Acad Sci U S A 105:3368-73 (2008). WB, IHC-FoFr ; Chicken . Read more (PubMed: 18299578) »

    See all 2 Publications for this product

    Product Wall

    Thanks for your call yesterday and for your patience. We have two HIF1AN antibodies that have been tested with mouse samples and are guaranteed to work in mouse: ab92304 and ab63163- http://www.abcam.com/HIF1AN-antibody-ab63163.html ...

    Read More
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293, Jurkat, Raw264)
    Loading amount 20 µg
    Specification HEK293, Jurkat, Raw264
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Feb 24 2009

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa and SK-N-SH)
    Specification HeLa and SK-N-SH
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Jul 24 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"