Anti-HIF2 alpha antibody (ab13704)

Overview

• Product nameAnti-HIF2 alpha antibodySee all HIF2 alpha primary antibodies ...
• Description
  Rabbit polyclonal to HIF2 alpha
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide, corresponding to amino acids 426-443 (AYGKAILPPSQPWATELR) of Human HIF2 alpha.

• Positive controlTested with HeLa cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism
  • Molecular processes
  • Mitochondrial transcription

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Cancer
  • Invasion/microenvironment
  • Hypoxia
  • HIF

  • Cardiovascular
  • Hypoxia
  • HIF

  • Epigenetics and Nuclear Signaling
  • Cardiovascular/Immune
  • Hypoxia
  • HIF

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • HLH

Applications

Target

• FunctionTranscription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
• Tissue specificityExpressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
• Involvement in diseaseDefects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.
• Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  Contains 1 PAC (PAS-associated C-terminal) domain.
  Contains 2 PAS (PER-ARNT-SIM) domains.
• Post-translational
  modificationsIn normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
  In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
  Phosphorylated on multiple sites in the CTAD.
  The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q99814 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2034 Human
  • Omim: 603349 Human
  • SwissProt: Q99814 Human
  • Unigene: 468410 Human

• Alternative names
  Basic helix loop helix PAS protein MOP2 antibodyBasic-helix-loop-helix-PAS protein MOP2 antibodybHLHe73 antibody

  Class E basic helix-loop-helix protein 73 antibodyECYT4 antibodyEndothelial PAS domain containing protein 1 antibodyEndothelial pas domain protein 1 antibodyEndothelial PAS domain-containing protein 1 antibodyEPAS 1 antibodyEPAS-1 antibodyEPAS1 antibodyEPAS1_HUMAN antibodyHIF 1 alpha like factor antibodyHIF 2 alpha antibodyHIF-1-alpha-like factor antibodyHIF-2-alpha antibodyHIF1 alpha like factor antibodyHIF2-alpha antibodyHif2a antibodyHLF antibodyHypoxia inducible factor 2 alpha antibodyHypoxia inducible factor 2 alpha subunit antibodyHypoxia-inducible factor 2-alpha antibodyMember of PAS protein 2 antibodyMember of pas superfamily 2 antibodyMOP 2 antibodyMOP2 antibodyPAS domain-containing protein 2 antibodyPASD2 antibody

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Anti-HIF2 alpha antibody images

• 

  - HIF2 alpha antibody (ab13704)

  Western blot analysis of HIF2 alpha using ab13704 at 1/500 dilution against (A) cobalt chloride treated HeLa cells and (B) untreated HeLa cell lysate.