![Western blot - HIF2 alpha antibody [ep190b] (ab8365) image](#ab8365.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HIF2 alpha antibody [ep190b] (ab8365) image](#HIF2-alpha-Primary-antibodies-ab8365-1.jpg)
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Product name
Anti-HIF2 alpha antibody [ep190b]
See all HIF2 alpha products (9) ...
Description
Mouse monoclonal [ep190b] to HIF2 alpha
Specificity
This antibody is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Tested applications
ELISA, IHC-Fr, IHC-P, WBmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Human EPAS-1 (HIF2A) amino acids 535-631 fusion protein with proprietary tag.
Positive control
Hypoxic A549 lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Ascitic fluid with 0.1% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
ep190b
Myeloma
NS1
Isotype
IgG1
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Nucleotide metabolism >> Molecular processes >> Mitochondrial transcription
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: 1/1000 - 1/3000.
WB: 1/100 - 1/500. Detects a band of approximately 118 kDa (predicted molecular weight: 100 kDa).
Target
Function
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Tissue specificity
Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
Involvement in disease
Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
Phosphorylated on multiple sites in the CTAD.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ99814
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic helix loop helix PAS protein MOP2 antibody
- Basic-helix-loop-helix-PAS protein MOP2 antibody
- bHLHe73 antibody
see all
Database links
- Entrez Gene: 2034 Human
- Entrez Gene: 29452 Rat
- Omim: 603349 Human
- SwissProt: Q99814 Human
- SwissProt: Q9JHS1 Rat
- Unigene: 468410 Human
- Unigene: 55138 Rat
Anti-HIF2 alpha antibody [ep190b] images:
Western blot - HIF2 alpha antibody [ep190b] (ab8365)
![Western blot - HIF2 alpha antibody [ep190b] (ab8365)](/ps/datasheet/Images/8/ab8365/ab8365.jpg)
Predicted band size : 100 kDa
HIF-2 alpha detected in hypoxic human lysate using ab8365. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HIF2 alpha antibody [ep190b] (ab8365)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HIF2 alpha antibody [ep190b] (ab8365)](/ps/datasheet/images/8/ab8365/HIF2-alpha-Primary-antibodies-ab8365-1.jpg)
ab8365 at 1/300 dilution, staining HIF2 alpha in cardiomyocytes of human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
References for Anti-HIF2 alpha antibody [ep190b] (ab8365)
This product has been referenced in:
- Koukourakis MI et al. Hypoxia inducible factor (HIF-1a and HIF-2a) expression in early esophageal cancer and response to photodynamic therapy and radiotherapy. Cancer Res 61:1830-2 (2001).Read more (PubMed: 11280732) »
- Bensellam M et al. Glucose-induced O2 consumption activates hypoxia inducible factors 1 and 2 in rat insulin-secreting pancreatic beta-cells. PLoS One 7:e29807 (2012). WB.Read more (PubMed: 22235342) »
See all 23 publications for this product
Publishing research using ab8365? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"