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Overview

  • Product nameHINT1 protein (Tagged-His Tag)See all HINT1 proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-126 of Human HINT1 with N terminal His tag, 126 amino acids, 15kDa. UniProt ID = P49773.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 62 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesMADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLA F HDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLM IVGK KCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVL GGRQMHWPPG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91690 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Adenosine 5' monophosphoramidaseAdenosine 5''-monophosphoramidaseHINT 1
        HINT1HINT1_HUMANHistidine triad nucleotide binding protein 1Histidine triad nucleotide-binding protein 1PKCI 1PKCI-1PKCI1PRKCNH1Protein kinase C inhibitor 1Protein kinase C interacting protein 1Protein kinase C-interacting protein 1
      see all
  • FunctionHydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the HINT family.
    Contains 1 HIT domain.
  • DomainThe histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
  • Cellular localizationCytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.

    • Target information above from: UniProt accession P49773 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    HINT1 protein (Tagged-His Tag) images

    • SDS-PAGE - HINT1 protein (Tagged-His Tag) (ab91690)
      SDS-PAGE - HINT1 protein (Tagged-His Tag) (ab91690)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for HINT1 protein (Tagged-His Tag) (ab91690)

    ab91690 has not yet been referenced specifically in any publications.

    Publishing research using ab91690 ? Please let us know so that we can cite the reference in this datasheet

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