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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab37917? Please let us know so that we can cite the reference in this datasheet
ab37917 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - HIP2 antibody (ab37917)
Anti-HIP2 antibody (ab37917) at 0.5 µg/ml + Jurkat lysate (RIPA buffer, 35µg total protein per lane)
Predicted band size : 23 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot - HIP2 antibody (ab37917)
Anti-HIP2 antibody (ab37917) at 1/1000 dilution + Cell lysate prepared from chicken DT40 lymphoblast cell line at 30 µg
Secondary
Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed (ab7125) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
Samples blocked with 5% milk for 30 minutes at room temperature.Gel 12.5-15% run under denaturing conditions.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP2 antibody (ab37917)
ab37917 at 3.7 µg/ml staining HIP2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
HIP2 antibody for WB in Human (37917)
HIP2 antibody for WB in Chicken (37917)
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