Anti-HIPK4 antibody (ab69565)
Key features and details
- Rabbit polyclonal to HIPK4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HIPK4 antibody -
Description
Rabbit polyclonal to HIPK4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthesized peptide derived from an internal internal of human HIPK4.
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Positive control
- A549 clls; HUVEC cells; testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 147746 Human
- Entrez Gene: 233020 Mouse
- Entrez Gene: 308449 Rat
- Omim: 611712 Human
- SwissProt: Q8NE63 Human
- SwissProt: Q3V016 Mouse
- SwissProt: Q4V793 Rat
- Unigene: 79363 Human
see all -
Alternative names
- HIPK 4 antibody
- HIPK4 antibody
- HIPK4_HUMAN antibody
see all
Images
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All lanes : Anti-HIPK4 antibody (ab69565) at 1/500 dilution
Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa -
Immunohistochemistry analysis of paraffin-embedded human testis tissue using 1:50 ab69565. For the right hand image the antibody was pre-incubated with the immunizing peptide.
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Immunofluorescence analysis of A549 cells, using 1:500 ab69565. For the right hand image, the antibody was pre-incubated with the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab69565 has not yet been referenced specifically in any publications.