![Western blot - HIRA antibody [374C6a] (ab70734) image](#ab70734.gif)
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Overview
Product name
Anti-HIRA antibody [374C6a]
See all HIRA products (4) ...
Description
Mouse monoclonal [374C6a] to HIRA
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment derived from internal sequence of human HIRA
Positive control
Recombinant human HIRA.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab70734 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Clonality
Monoclonal
Clone number
374C6a
Isotype
IgG2a
Research areas
Developmental Biology >> Reproduction >> Germ cell markers
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> RNAi >> RISC
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Western blot - HIRA antibody [374C6a] (ab70734)
![Western blot - HIRA antibody [374C6a] (ab70734) image](/ps/datasheet/Images/70/ab70734/ab70734.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 112 kDa.
Dot: Use at an assay dependent dilution.
Target
Relevance
The HIRA gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in flies, yeast and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Cellular localization
Nuclear
Alternative names
- DGGR 1 antibody
- DiGeorge critical region gene 1 antibody
- HIR (histone cell cycle regulation defective S cerevisiae) homolog A antibody
see all
Database links
- Entrez Gene: 7290 Human
- SwissProt: P54198 Human
- Unigene: 474206 Human
Anti-HIRA antibody [374C6a] images:
Western blot - HIRA antibody [374C6a] (ab70734)
Anti-HIRA antibody [374C6a] (ab70734) + immunising recombinant protein
Predicted band size : 112 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
References for Anti-HIRA antibody [374C6a] (ab70734)
ab70734 has not yet been referenced specifically in any publications.
Publishing research using ab70734? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"