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RabMAbs (5658-1)

Anti-HIRA antibody [EPR7416] (ab129169)

Submit an Abreview Q&A (9)

Overview

  • Product nameAnti-HIRA antibody [EPR7416]See all HIRA primary antibodies ...
  • Description
    Rabbit monoclonal [EPR7416] to HIRA
  • Tested applicationsWB, IP, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human HIRA.

  • Positive control293T, HeLa and K562 cell lysates, HeLa cells
  • General notesProduced under U.S. Patent No. 5,675,063

Properties

Applications

Our Abpromise guarantee covers the use of ab129169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Predicted molecular weight: 112 kDa.
IP IP: 1/10 - 1/100.
Flow Cyt Flow Cyt: 1/100 - 1/500.
ICC/IF ICC/IF: 1/50 - 1/100.
  • Application notesIs unsuitable for or IHC-P.

    • Target

    • FunctionCooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
    • Tissue specificityExpressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
    • Sequence similaritiesBelongs to the WD repeat HIR1 family.
      Contains 8 WD repeats.
    • Developmental stageExpressed during embryogenesis.
    • Post-translational
      modifications      Sumoylated.
      Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
    • Cellular localizationNucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.

      • Target information above from: UniProt accession P54198 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          DGCR1 antibodyDiGeorge critical region gene 1 antibodyHIR antibody
          HIR histone cell cycle regulation defective homolog A antibodyHIRA antibodyHIRA protein antibodyHIRA_HUMAN antibodyProtein HIRA antibodyTUP 1 antibodyTUP1 like enhancer of split protein 1 antibodyTUP1-like enhancer of split protein 1 antibodyTUPLE1 antibody
        see all

      Anti-HIRA antibody [EPR7416] images

      • Western blot - Anti-HIRA antibody [EPR7416] (ab129169)
        Western blot - Anti-HIRA antibody [EPR7416] (ab129169)
        All lanes : Anti-HIRA antibody [EPR7416] (ab129169) at 1/1000 dilution

        Lane 1 : 293T cell lysates
        Lane 2 : HeLa cell lysates
        Lane 3 : K562 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

        Predicted band size : 112 kDa
      • Immunocytochemistry/ Immunofluorescence - Anti-HIRA antibody [EPR7416] (ab129169)
        Immunocytochemistry/ Immunofluorescence - Anti-HIRA antibody [EPR7416] (ab129169)
        ab129169, at 1/50 dilution, staining HIRA in HeLa cells by Immunofluorescence.
      • Other-Anti-HIRA antibody [EPR7416](ab129169)
        Other-Anti-HIRA antibody [EPR7416](ab129169)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References for Anti-HIRA antibody [EPR7416] (ab129169)

      ab129169 has not yet been referenced specifically in any publications.

      Publishing research using ab129169 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab129169 Submit a Question for ab129169

      Displaying 1 - 9 of 9 results for Abreviews and Q&A

      10
      Votes: Highest First

      The permeabilization with NP40 is sufficient, as I can detect nuclear viral proteins using our protocol.

      Thank you for that confirmation. I wanted to be sure you were confident the NP40 was sufficient, since other antibodies will give the same result if it is not.

      I can send a replacement but we do not have an alternative lot of ab129169 and I am ...

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      We used paraformaldehyde to fix HeLa cells and NP40 to permeabilize. I blocked the samples in 5% normal goat serum overnight and stained with the anti HIRA antibody at 1/50 for 1hr. My secondary antibodies were functional, as other antibodies done in para...

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      Thank you for the extra details of your protocol. You should be seeing a nuclear signal from this cell line, if the permeabilization is sufficient. Have you been able to stain other nuclear proteins with this protocol? We recommend fixation for no more th...

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      We recently ordered a Anti-HIRA antibody from abcam (ab129169) and have found that it does not work as advertised for IF. I used the antibody at the recommended dilution of 1/50 and saw no specific staining with the antibody. We would like this purchase t...

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      A replacement or refund for anti-HIRA ab129169 is available, assuming the species of your samples is human and not mouse or rat, which we list on the datasheet as non-reactive.

      For complaints, we also routinely ask for a few details of the prot...

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      Thank you for your detail information. It is useful and good to know that. Then I would like to try the anti-antibody (ab20555) which you recommended.Thank you very much

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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      I definitely want to give it (ab20655) a shot. However, do you know why the predicted size of HIRA is different detected by two different antibodies? In the data sheet, the HIRA detected by ab20555 is 89 kDa, while it is 112 kDa byAb129169.



      I have checked SwissProte, and it seems that there are two isoforms: one with 112kDa, and one with 89 kDa, which would match perfectly. You canfind the database link on ourdatasheet.

      In addition, I found that we received almost theexac...

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      The lysate came from HeLa cells and the HIRA is endogenous protein. I did not overexpress HIRA in these cells. I did denature protein at 95ºC with beta-ME and use a reducing gel. I also used your anti-H2B and anti-NAP1L1 antibody to detect the same c...

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      As I said before, and your email is confirming this, I am afraid that your protocol is all right, but for an unknown reason this two last vials did not work. And yes, they are from thesame batch, but different aliquots.

      I have flagged this ...

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      I did use the second batch of anti-HIRA antibody you provided to me last week. However, its quality is still poor. I attached the questionnaire form and figure I did last Thursday below. Hope you can help me to solve the problem.

      Thank you for contacting us again.

      I am going to suggest you a few things which used in combination usually work to lower the background. However, I am slightly concerned that your results look so very non-specific.

      1. Measure the pro...

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      1) Abcam product code ab
      Ab129169


      3) Description of the problem
      Detecting multiple non-specific bands

      4) Sample preparation:
      Type of sample (whole cell lysates, fraction, recombinant protein…)...

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      Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

      The details provided will enable us to investigate this case and will provide us with vital i...

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      I ordered an anti-HIRA antibody (ab129169) on April 20, got them last week and used it this week for western blot (1:1000 dilution). However, I found multiple nonspecific bands on the blot and can not tell which band is HIRA. The antibody is not performin...

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      Thank you for contacting us.

      I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      I am a...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"