Anti-HIRA antibody (ab20655)

Thank you for your detail information. It is useful and good to know that. Then I would like to try the anti-antibody (ab20555) which you recommended.Thank you very much

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 11111111111.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

I definitely want to give it (ab20655) a shot. However, do you know why the predicted size of HIRA is different detected by two different antibodies? In the data sheet, the HIRA detected by ab20555 is 89 kDa, while it is 112 kDa byAb129169.

ANSWER:

I have checked SwissProte, and it seems that there are two isoforms: one with 112kDa, and one with 89 kDa, which would match perfectly. You canfind the database link on ourdatasheet.

In addition, I found that we received almost theexact same question in 2006, and here is what my colleague answered: "I have been in touch with my chromatin business development colleague and the size of the band that this antibody detects (89KDa) can be explained by the splice variant shown at the following SwissProt link:

http://us.expasy.org/uniprot/P54198-2

At present we have not been able to detect full length HIRA: 112KDa as defined by the SwissProt reference P54198."

This answer can be found within the Scientific Support Tag on our datasheet.

Please let me know if you still want to try the ab20655 Anti-HIRA antibody.

Dear Sir/Madam,

I am interested in having an antibody against HIRA. I have found your HIRA antibody (ab20655), but I slightly confused because on the picture shown in your website, the size of the bands detected don't match the size of published HIRA.

Am I missing something? Please let me know if you can help.

Many thanks.

Best regards.

ANSWER:

Thank you for your enquiry.

I have been in touch with my chromatin business development colleague and the size of the band that this antibody detects (89KDa) can be explained by the splice variant shown at the following SwissProt link:

http://us.expasy.org/uniprot/P54198-2

At present we have not been able to detect full length HIRA: 112KDa as defined by the SwissProt reference P54198.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Dear Sir/Madam,

I am interested in having an antibody against HIRA. I have found your HIRA antibody (ab20655), but I slightly confused because on the picture shown in your website, the size of the bands detected don't match the size of published HIRA.

Am I missing something? Please let me know if you can help.

ANSWER:

Thank you for your enquiry.

Ab20655 recognises a band at 89kDa which corresponds to the short isoform of HIRA. This band was specifically blocked by the immunising peptide.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.