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Anti-HIRA antibody (ab20655)

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Overview

Product name

Anti-HIRA antibody
See all HIRA products (4) ...

Description

Rabbit polyclonal to HIRA

Specificity

ab20655 recognises a band of 89kDa corresponding to the short isoform of HIRA in HeLa, HeLa Nuclear, A431 and HEK293 lysates.

Tested applications

ICC/IF, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat, Dog

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human HIRA.

Read Abcam's proprietary immunogen policy

(Peptide available as ab22456.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Developmental Biology >> Reproduction >> Germ cell markers
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> RNAi >> RISC
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi

  • Western blot - HIRA antibody (ab20655)Western blot - HIRA antibody (ab20655) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab20655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA (predicted molecular weight: 89 kDa). Can be blocked with HIRA peptide (ab22456).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The HIRA gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in flies, yeast and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Cellular localization

Nuclear

Alternative names

  • DGGR 1 antibody
  • DGGR1 antibody
  • DiGeorge critical region gene 1 antibody
  • HIR antibody
  • HIR histone cell cycle regulation defective homolog A antibody
  • HIRA protein antibody
  • TUP 1 antibody
  • TUP1 antibody
  • TUP1 like enhancer of split protein 1 antibody
  • TUPLE 1 antibody
  • TUPLE1 antibody
see all

Database links

Anti-HIRA antibody images:

  Western blot - HIRA antibody (ab20655)

Western blot - HIRA antibody (ab20655)

Anti-HIRA antibody (ab20655) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660) at 10 µg

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 89 kDa
Observed band size : 89 kDa
Additional bands at : 37 kDa (possible cross reactivity).

ab20655 recognises a band at 89kDa, corresponding to the short isoform of HIRA.

References for Anti-HIRA antibody (ab20655)

This product has been referenced in:

  • Lewis PWet al. Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeres. Proc Natl Acad Sci U S A 107:14075-80 (2010). ChIP; Mouse.Read more (PubMed: 20651253) »
  • Dutta Det al. Regulation of angiogenesis by histone chaperone HIRA-mediated incorporation of lysine 56-acetylated histone H3.3 at chromatin domains of endothelial genes. J Biol Chem 285:41567-77 (2010). WB; Mouse.Read more (PubMed: 21041298) »

See all 2 publications for this product

Publishing research using ab20655? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"