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ab22456 |
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ab22456 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Anti-HIRA antibody
See all HIRA products (4) ...
Rabbit polyclonal to HIRA
ab20655 recognises a band of 89kDa corresponding to the short isoform of HIRA in HeLa, HeLa Nuclear, A431 and HEK293 lysates.
ICC/IF, WBmore details
Reacts with
Mouse, Human
Predicted to work with
Rat, Dog
Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human HIRA.
(Peptide available as ab22456.)
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Developmental Biology >> Reproduction >> Germ cell markers
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> RNAi >> RISC
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Western blot - HIRA antibody (ab20655)
(enlarge)
Our Abpromise guarantee covers the use of ab20655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA (predicted molecular weight: 89 kDa). Can be blocked with HIRA peptide (ab22456).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The HIRA gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in flies, yeast and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Nuclear
Western blot - HIRA antibody (ab20655)

Anti-HIRA antibody (ab20655) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 89 kDa
Additional bands at : 37 kDa (possible cross reactivity).
ab20655 recognises a band at 89kDa, corresponding to the short isoform of HIRA.
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab20655? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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Anti-HIRA antibody (ab20655) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 89 kDa
Additional bands at : 37 kDa (possible cross reactivity).
ab20655 recognises a band at 89kDa, corresponding to the short isoform of HIRA.
2
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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