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Western blot - HIRA antibody (ab20655)
(enlarge)
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Product Name
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HIRA antibody
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See all HIRA antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HIRA
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human HIRA.
(Peptide available as ab22456.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Dog
(Due to sequence homology)
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Specificity
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ab20655 recognises a band of 89kDa corresponding to the short isoform of HIRA in HeLa, HeLa Nuclear, A431 and HEK293 lysates.
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA (predicted molecular weight: 89 kDa). Can be blocked with HIRA peptide (ab22456).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Reproduction >> Germ cell markers
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Chromatin and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Chromatin and Nuclear Signaling >> RNAi >> RISC
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Relevance
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The HIRA gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in flies, yeast and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.40 - 0.40 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HIRA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HIRA antibody images, references, products related to ab20655 and other tools.
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HIRA antibody images:
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 Western blot - HIRA antibody (ab20655)
HIRA antibody (ab20655) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 89 kDa
Additional bands at : 37 kDa (possible cross reactivity).
ab20655 recognises a band at 89kDa, corresponding to the short isoform of HIRA.
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Search PubMed (MEDLINE) for references to HIRA
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HIRA antibody - more information
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Customer reviews (feedback) regarding HIRA antibody |
Customer FAQs regarding HIRA antibody |
Protocols for HIRA antibody |
Price and availability of products related to HIRA antibody |
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HIRA antibody - related products:
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HIRA antibody - other tools:
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Contact Abcam with a Technical Enquiry about HIRA antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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