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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab22456 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Blocking - HIRA peptide (ab22456)
All lanes : HIRA antibody (ab20655) at 1 ug/ml
Lane 1 : HeLa Nuclear Lysate
Lane 2 : HeLa Nuclear Lysate with HIRA peptide (ab22456) at 1 ug/ml
Lysates/proteins at 20 ug per lane.
Predicted band size : 89kDa
Observed band size : 89kDa
Additional bands at : 32kDa (possible cross reactivity).
ab20655 recognises a band at 89kDa, corresponding to the short isoform of HIRA (lane 1). This band is specifically blocked by the immunising peptide, ab22456 (lane 2). There is a band at 32kDa, which is not blocked by the immunising peptide and this is attributed to cross-reactivity.
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