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Overview
Product name
HIRA peptide
Protein description
Synthetic peptide derived from within residues 1000 to the C-terminus of Human HIRA. (Note: the amino acid sequence is proprietary) This peptide was used as an immunogen for ab20655.
Protein length
1017 amino acids
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
Research areas
Developmental Biology >> Reproduction >> Germ cell markers
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> RNAi >> RISC
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Blocking - HIRA peptide (ab22456)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
Blocking
Application notes
BL: Use at a concentration of 1 µg/ml.
This peptide can be used with studies using ab20655.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
The HIRA gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in flies, yeast and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Cellular localization
Nuclear
HIRA peptide images:
Blocking - HIRA peptide (ab22456)
All lanes : HIRA antibody (ab20655) at 1 ug/ml
Lane 1 : HeLa Nuclear Lysate
Lane 2 : HeLa Nuclear Lysate with HIRA peptide (ab22456) at 1 ug/ml
Lysates/proteins at 20 ug per lane.
Predicted band size : 89kDa
Observed band size : 89kDa
Additional bands at : 32kDa (possible cross reactivity).
ab20655 recognises a band at 89kDa, corresponding to the short isoform of HIRA (lane 1). This band is specifically blocked by the immunising peptide, ab22456 (lane 2). There is a band at 32kDa, which is not blocked by the immunising peptide and this is attributed to cross-reactivity.
References for HIRA peptide (ab22456)
ab22456 has not yet been referenced specifically in any publications.
Publishing research using ab22456? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"