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Blocking - HIRA peptide (ab22456)
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Product Name
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HIRA peptide
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Product type
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Proteins and Peptides
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Peptide
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Synthetic peptide derived from within residues 1000 to the C-terminus of Human HIRA.
This peptide was used as an immunogen for ab20655.
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Tested applications
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BL
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Application notes
(see key)
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Recommended dilutions
BL: Use at a concentration of 1 µg/ml.
This peptide can be used with studies using ab20655.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Developmental Biology >> Reproduction >> Germ cell markers
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Chromatin and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Chromatin and Nuclear Signaling >> RNAi >> RISC
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Relevance
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The HIRA gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in flies, yeast and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
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Storage buffer
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Preservative: None
Constituents: 0.001% Tween 20, HEPES, 2mM EDTA, 150mM Sodium chloride
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HIRA peptide is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HIRA peptide images, references, products related to ab22456 and other tools.
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HIRA peptide images:
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 Blocking - HIRA peptide (ab22456)
All lanes : HIRA antibody (ab20655) at 1 ug/ml
Lane 1 : HeLa Nuclear Lysate
Lane 2 : HeLa Nuclear Lysate with HIRA peptide (ab22456) at 1 ug/ml
Lysates/proteins at 20 ug per lane.
Predicted band size : 89kDa
Observed band size : 89kDa
Additional bands at : 32kDa (possible cross reactivity).
ab20655 recognises a band at 89kDa, corresponding to the short isoform of HIRA (lane 1). This band is specifically blocked by the immunising peptide, ab22456 (lane 2). There is a band at 32kDa, which is not blocked by the immunising peptide and this is attributed to cross-reactivity.
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Search PubMed (MEDLINE) for references to HIRA
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HIRA peptide - more information
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Customer reviews (feedback) regarding HIRA peptide |
Customer FAQs regarding HIRA peptide |
Protocols for HIRA peptide |
Price and availability of products related to HIRA peptide |
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HIRA peptide - related products:
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HIRA peptide - other tools:
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Contact Abcam with a Technical Enquiry about HIRA peptide |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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