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Overview
Product name
Anti-HIRA (phospho T555) antibody
See all HIRA products (4) ...
Description
Rabbit polyclonal to HIRA (phospho T555)
Specificity
ab61095 detects endogenous levels of HIRA only when phosphorylated at threonine 555.
Tested applications
ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthesized phosphopeptide derived from human HIRA around the phosphorylation site of threonine 555 (L-T-TP-P-S).
Positive control
HeLa cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Germ cell markers
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> RNAi >> RISC
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Immunocytochemistry/ Immunofluorescence - HIRA (phospho T555) antibody (ab61095)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab61095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500 - 1/1000.
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Relevance
The HIRA gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in flies, yeast and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Cellular localization
Nuclear
Alternative names
- DGGR 1 antibody
- DGGR1 antibody
- DiGeorge critical region gene 1 antibody
see all
Database links
- Entrez Gene: 7290 Human
- Entrez Gene: 15260 Mouse
- SwissProt: P54198 Human
- SwissProt: Q61666 Mouse
- Unigene: 474206 Human
Anti-HIRA (phospho T555) antibody images:
Immunocytochemistry/ Immunofluorescence - HIRA (phospho T555) antibody (ab61095)
Immunofluorescence analysis of HeLa cells, using ab61095 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).
References for Anti-HIRA (phospho T555) antibody (ab61095)
ab61095 has not yet been referenced specifically in any publications.
Publishing research using ab61095? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"