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Overview

  • Product nameAnti-HIST1H2BD antibody
  • Description
    Rabbit polyclonal to HIST1H2BD
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 1-30 of Human HIST1H2BD (NP_619790.1; NP_066407.1).

  • Positive controlHL60 cell line lysates.

Properties

  • FormLiquid
  • Storage instructionsStore at 2-8°C for up to 6 months. For long term storage store at -20°C.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab129624 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab129624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/100 - 1/500. Predicted molecular weight: 14 kDa.

Target

  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similaritiesBelongs to the histone H2B family.
  • Post-translational
    modifications    Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
    Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
  • Cellular localizationNucleus. Chromosome.

    • Target information above from: UniProt accession P58876 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        dJ221C16.6 antibodyH2B histone family, member B antibodyH2B.1B antibody
        H2B/b antibodyH2B1D_HUMAN antibodyH2bd antibodyH2BFB antibodyHIRA-interacting protein 2 antibodyHIRIP2 antibodyHIST1H2BD antibodyhistone cluster 1, H2bd antibodyHistone H2B type 1-D antibodyHistone H2B.1 B antibodyHistone H2B.b antibody
      see all

    Anti-HIST1H2BD antibody images

    • Western blot - Anti-HIST1H2BD antibody (ab129624)
      Western blot - Anti-HIST1H2BD antibody (ab129624)
      Anti-HIST1H2BD antibody (ab129624) at 1/100 dilution + HL60 cell line lysates at 35 µg
      developed using the ECL technique

      Predicted band size : 14 kDa

    References for Anti-HIST1H2BD antibody (ab129624)

    ab129624 has not yet been referenced specifically in any publications.

    Publishing research using ab129624 ? Please let us know so that we can cite the reference in this datasheet

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