Recombinant
RabMAb

Anti-HIV TAT specific factor 1 antibody [EPR9104(B)] (ab140654)

Overview

  • Product name
    Anti-HIV TAT specific factor 1 antibody [EPR9104(B)]
    See all HIV TAT specific factor 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9104(B)] to HIV TAT specific factor 1
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HIV TAT specific factor 1 aa 1-100.

  • Positive control
    • Jurkat and HeLa cell lysates; Human colon and uterus tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab140654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 86 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance
      HIV TAT specific factor(a.k.a. HTATSF1, Tat-SF1 or HTSF1) is an 86 kDa general transcription factor that plays a role in the process of transcription elongation. However, in HIV-infected cells, this factor is up-regulated by HIV Nef and gp120 and acts as a co-factor for the Tat-enhanced transcription of the HIV virus.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • Cofactor required for Tat activation of HIV 1 transcription antibody
      • dJ196E23.2 antibody
      • HIV Tat specific factor 1 antibody
      • HTATSF1 antibody
      • HTSF-1 antibody
      • HTSF1 antibody
      • TAT SF1 antibody
      • Tat-SF1 antibody
      see all

    Images

    • All lanes : Anti-HIV TAT specific factor 1 antibody [EPR9104(B)] (ab140654) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 86 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HIV TAT specific factor 1 with ab140654 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue labelling HIV TAT specific factor 1 with ab140654 at 1/250 dilution.
    • Immunofluorescent analysis of HeLa cells labelling HIV TAT specific factor 1 with ab140654 at 1/250 dilution.

    References

    ab140654 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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