Overview

  • Product nameAnti-HIV protease antibody [1696]
  • Description
    Mouse monoclonal [1696] to HIV protease
  • SpecificityThe antibody recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2). Does not react with precursor.
  • Tested applicationsWB, ELISA, Inhibition Assaymore details
  • Immunogen

    Recombinant HIV-1 protease.

Properties

Applications

Our Abpromise guarantee covers the use of ab8327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent dilution. Strongly inhibits the activity of HIV-1 and HIV-2 proteases.

Target

  • RelevanceThe HIV1 core consists of a viral genome housed within a conical viral capsid that is generated during virion maturation. Human immunodeficiency virus type 1 (HIV1) matures after the viral protease processes the Gag and Pol polyproteins at 10 substrate locations. The protease of HIV1 is an aspartic protease and is functional only as a dimer; dimerization results in the formation of a binding cleft in which each of the two catalytic aspartic acids in which each monomer contributes each of the 2 catalytic aspartic acids. Because the protease is active only as a dimer, two of the GagPol precursors must themselves dimerize during virus assembly so that their protease domains can dimerize, become active, and process the precursors. Both the order and kinetics of cleavage as well as the extent of precursor processing appear to be critical steps in the generation of fully infectious, appropriately assembled viral particles. Inhibition of HIV-1 protease represents an important avenue for antiviral therapy. Currently available combination chemotherapy with reverse transcriptase inhibitors (RTIs) and protease inhibitors (PIs) for human immunodeficiency virus type 1 (HIV1) infection and AIDS have been shown to suppress the replication of HIV1 and extend the life expectancy of HIV1 infected individuals.
  • Alternative names
    • HIV-1 protease antibody
    • Human immunodeficiency virus protease antibody
    • PR antibody
    • Retropepsin antibody

References for Anti-HIV protease antibody [1696] (ab8327)

This product has been referenced in:
  • O'loughlin TL  et al. Diversification and Specialization of HIV Protease Function During In Vitro Evolution. Mol Biol Evol 23:764-72 (2006). WB . Read more (PubMed: 16423863) »
  • Lescar J  et al. Inhibition of the HIV-1 and HIV-2 proteases by a monoclonal antibody. Protein Sci 8:2686-96 (1999). Read more (PubMed: 10631984) »

See all 2 Publications for this product

Product Wall

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We have anti HIV protease antibodies avialable in catalogue. Please contact our indian distributor for price and shipment;

Allied Scientific Products
39/19B Gopal Nagar Road
Kolkata-700027
Indi...

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Thank you for contacting us.

I have good news:

The supplying lab let me know the following:

We are able to produce Fab fragments from HIV protease antibody [clone1696].
The minimun amount of the HIV protease must be ...

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I would suggest that you should carry out a preliminary experiment with different dilutions of this antibody (i.e.1:500-1:2000) and then adjust the dilution accordingly from the results obtained.

This antibody has not been tested against the mutant forms of HIV protease so we do not know whether or not it would react with them.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"