Overview
Product nameHIV1 Nef proteinSee all HIV1 Nef proteins and peptides ...
Protein descriptionFull length recombinant protein, from HIV1, subtype B, MW 27kDa. Genbank AAA44988.1. This peptide was used as an immunogen for ab63918 - .
Expression hostE. coli
Properties
Purity> 90
% by SDS-PAGE
Purification notesHighly purified by several steps of chromatography.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 50mM Sodium chloride, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 7.5
Concentration information loading...
Additional notesab63996 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab63996
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
-
Alternative names
C terminal core proteinF proteinNef
see all
RelevanceNef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
References for HIV1 Nef protein (ab63996)
ab63996
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - HIV1 Nef protein (ab63996)Polyacrylamide gel electrophoresis of ab63996.