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Products:Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
Overview
Product name
Anti-HIV1 Reverse Transcriptase antibody [8C4D7]
See all HIV1 Reverse Transcriptase products (5) ...
Description
Mouse monoclonal [8C4D7] to HIV1 Reverse Transcriptase
Specificity
Binds to recombinant Reverse Transcriptase HIV1 (IIIB).
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with HIV1,(IIIB).
Immunogen
Purified full length recombinant HIV1 Reverse Transcriptase produced in E. coli Expression system.
Properties
Form
Lyophilised:
Reconstitute with distilled water
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified IgG, >95% pure by SDS-PAGE analysis. Not tested for sterility or endotoxin content.
Clonality
Monoclonal
Clone number
8C4D7
Isotype
IgG1
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ELISA
Application notes
ELISA: 1/1000 (when antibody concentration is 1 mg/ml)
WB: 1/500 - 1/1000 (when antibody concentration is 1 mg/ml).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
Alternative names
- HIV 1 Reverse transcriptase antibody
- HIV1 RT antibody
- Human Immunodeficiency virus 1 antibody
see all
References for Anti-HIV1 Reverse Transcriptase antibody [8C4D7] (ab87398)
This product has been referenced in:
- Hehl EAet al. Interaction between human immunodeficiency virus type 1 reverse transcriptase and integrase proteins. J Virol 78:5056-67 (2004).Read more (PubMed: 15113887) »
See 1 publication for this product
Publishing research using ab87398? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"