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Products:Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
Overview
Product name
Anti-HIV1 Reverse Transcriptase antibody
See all HIV1 Reverse Transcriptase products (5) ...
Description
Guinea pig polyclonal to HIV1 Reverse Transcriptase
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with HIV1.
Immunogen
HIV1 Reverse Transcriptase, over-expressed as recombinant protein in E.coli.
Positive control
Extract of MT4 cells infected with HIV-1 (LAI strain).
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
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Western blot - HIV1 Reverse Transcriptase antibody (ab63912)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
WB: 1/2500. Detects bands of approximately 51 and 66 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
Alternative names
- HIV 1 Reverse transcriptase antibody
- HIV 1 RT antibody
- HIV RT antibody
see all
Anti-HIV1 Reverse Transcriptase antibody images:
Western blot - HIV1 Reverse Transcriptase antibody (ab63912)
All lanes : Anti-HIV1 Reverse Transcriptase antibody (ab63912) at 1/2500 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)
Observed band size : 51,66 kDa (why is the actual band size different from the predicted?)
ab63912 detects bands of 66 kDa and 51 kDa corresponding to the two subunits of HIV1 reverse transcriptase.
References for Anti-HIV1 Reverse Transcriptase antibody (ab63912)
ab63912 has not yet been referenced specifically in any publications.
Publishing research using ab63912? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"