Anti-HIV1 p17 antibody [2D11] (ab9067)

Overview

• Product nameAnti-HIV1 p17 antibody [2D11]See all HIV1 p17 primary antibodies ...
• Description
  Mouse monoclonal [2D11] to HIV1 p17
• SpecificityReacts with Human Immunodeficiency Virus Type 1 (HIV 1) p17 protein
• Tested applicationsFlow Cyt, WB, ELISA, ICC/IF, Radioimmunoprecipitation assay more details
• EpitopeConformational epitope (i.e., pepscan negative)

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Purification notesPurified from serum-free culture supernatant.
• Primary antibody notes The antibody may be used in indirect immunostaining techniques to detect HIV-1 core protein in fresh or cultured HIV-1 infected cells. Studies on core antigen synthesis and metabolism are performed using Western blotting or radioimmunoprecipitation analysis. The antibody may also be valuable for the affinity isolation of HIV-1 core protein.
• Clonality Monoclonal
• Clone number2D11
• IsotypeIgG1
• Research Areas
  • Microbiology
  • Organism
  • Virus
  • RNA Virus
  • ssRNA positive strand virus
  • HIV

  • Immunology
  • Immune System Diseases
  • Antiviral Signaling
  • HIV

Applications

Target

• RelevanceHIV1 p17 is the matrix protein of the Gag polyprotein which performs highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
• Cellular localizationMatrix protein of HIV1 Gag polyprotein

• Alternative names
  HIV1 matrix protein p17 antibodyHIV1 p17 antibodyMA antibodyMatrix protein p17 antibody

Anti-HIV1 p17 antibody [2D11] images

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  Flow Cytometry - Anti-HIV1 p17 antibody [2D11] (ab9067)Image from Campbell GR & Spector SA, PLoS Pathog. 2012 May;8(5):e1002689. Epub 2012 May 10. Fig 1.; doi:10.1371/journal.ppat.1002689; May 10, 2012, PLoS Pathog 8(5): e1002689.
  Flow cytometric analysis of monocyte-derived macrophages infected with HIV, staining HIV1 p17 with ab9067. Cells were treated with 1a,25-dihydroxycholecalciferol (1,25D3) at varying concentrations, before being infected with HIV.
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  Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p17 antibody [2D11] (ab9067)Image from Campbell GR & Spector SA, J Biol Chem. 2011 May 27;286(21):18890-902. Epub 2011 Mar 30. Fig 6.; doi: 10.1074/jbc.M110.206110; May 27, 2011 The Journal of Biological Chemistry, 286, 18890-18902.
  Immunofluorescence analysis of Macrophages treated with 100 pM 1,25D3 or vehicle control (EtOH) and infected with HIV1_Ba-L, staining HIV1 p17 with ab9067.
  
  Cells were fixed and permeabilized in PBS supplemented with paraformaldehyde and 0.1% saponin for 30 min. Samples were incubated with primary antibody and an AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody.