Anti-HIV1 p17 antibody [32/5.8.42] (ab9064)

Overview

  • Product name
    Anti-HIV1 p17 antibody [32/5.8.42]
    See all HIV1 p17 primary antibodies
  • Description
    Mouse monoclonal [32/5.8.42] to HIV1 p17
  • Tested applications
    Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IFmore details
  • Immunogen

    Other Immunogen Type corresponding to HIV1 p17. ab9064 was raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate.

  • Epitope
    The antibody epitope has been mapped to amino acids 12-19 (see PMID: 2462060).
  • General notes

    The antibody may be used in indirect immunostaining techniques to detect HIV-1 core protein in fresh or cultured HIV-1 infected cells. Studies on core antigen synthesis and metabolism are performed using Western blotting or radioimmunoprecipitation analysis. The antibody may also be valuable for the affinity isolation of HIV-1 core protein.

    Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Phosphate buffered saline
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified from serum-free culture supernatant.
  • Primary antibody notes
    The antibody may be used in indirect immunostaining techniques to detect HIV-1 core protein in fresh or cultured HIV-1 infected cells. Studies on core antigen synthesis and metabolism are performed using Western blotting or radioimmunoprecipitation analysis. The antibody may also be valuable for the affinity isolation of HIV-1 core protein.
  • Clonality
    Monoclonal
  • Clone number
    32/5.8.42
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml.
Radioimmunoprecipitation Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 - 10 µg/ml.
AP Use at an assay dependent dilution.

Target

  • Relevance
    HIV1 p17 is the matrix protein of the Gag polyprotein which performs highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
  • Cellular localization
    Matrix protein of HIV1 Gag polyprotein
  • Alternative names
    • HIV1 matrix protein p17 antibody
    • HIV1 p17 antibody
    • MA antibody
    • Matrix protein p17 antibody

References

ab9064 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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