![Immunohistochemistry (Frozen sections) - HIV1 p24 antibody [38/8.7.47] (ab9044) image](#ab9044-1.jpg)
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Products:Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
Overview
Product name
Anti-HIV1 p24 antibody [38/8.7.47]
See all HIV1 p24 products (28) ...
Description
Mouse monoclonal [38/8.7.47] to HIV1 p24
Specificity
This murine monoclonal antibody reacts with Human Immunodeficiency Virus Type 1(HIV 1) p24 protein.
Tested applications
Radioimmunoprecipitation assay, WB, ELISA, ICC/IFmore details
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Phosphate buffered saline
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Purified from serum-free culture supernatant.
Primary antibody notes
The antibody is used to detect HIV-1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis.
Clonality
Monoclonal
Clone number
38/8.7.47
Isotype
IgG1
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
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Immunohistochemistry (Frozen sections) - HIV1 p24 antibody [38/8.7.47] (ab9044)
![Immunohistochemistry (Frozen sections) - HIV1 p24 antibody [38/8.7.47] (ab9044) image](/ps/datasheet/Images/9/ab9044/ab9044-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab9044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Radioimmunoprecipitation assay
WB
ELISA
ICC/IF
Application notes
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 1 - 10 µg/ml.
RipA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 10 µg/ml.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
Cellular localization
Membrane
Alternative names
- CA antibody
- Capsid protein p24 antibody
- Human immunodeficiency virus 1 antibody
- Human immunodeficiency virus type 1 p24 antibody
Anti-HIV1 p24 antibody [38/8.7.47] images:
Immunohistochemistry (Frozen sections) - HIV1 p24 antibody [38/8.7.47] (ab9044)
The images shows HIVADA-infected PBMC in vitro stained with ab9044. These images were kindly supplied as part of the review submitted by Eliseo Eugenin.
References for Anti-HIV1 p24 antibody [38/8.7.47] (ab9044)
This product has been referenced in:
- Maamary Jet al. Newcastle disease virus expressing a dendritic cell-targeted HIV gag protein induces a potent gag-specific immune response in mice. J Virol 85:2235-46 (2011). WB.Read more (PubMed: 21159873) »
- François KO & Balzarini J The Highly Conserved Glycan at Asparagine 260 of HIV-1 gp120 Is Indispensable for Viral Entry. J Biol Chem 286:42900-10 (2011). WB.Read more (PubMed: 22006924) »
See all 3 publications for this product
Publishing research using ab9044? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"