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Anti-HIV1 p24 antibody [39/5.4A] (ab9071)

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Overview

Product name

Anti-HIV1 p24 antibody [39/5.4A]
See all HIV1 p24 products (28) ...

Description

Mouse monoclonal [39/5.4A] to HIV1 p24

Specificity

Human Immunodeficiency Virus Type 1(HIV 1) p24 protein.

Tested applications

WB, Radioimmunoprecipitation assay, ELISA, ICC/IFmore details

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Purified from serum-free culture supernatant.

Primary antibody notes

The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. Useful as solid phase for capture of p24 in an antigen ELISA format.

Clonality

Monoclonal

Clone number

39/5.4A

Isotype

IgG1

Research areas

Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV

  • Western blot - HIV1 p24 antibody [39/5.4A] (ab9071)Western blot - HIV1 p24 antibody [39/5.4A] (ab9071) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab9071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

    Five stars (1 Abreview) WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 10 µg/ml.(Reactive with viral lysates . In addition, the antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts.)

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    Radioimmunoprecipitation assay

     RipA: Use at an assay de...Read more →

    RipA: Use at an assay dependent dilution. (The antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts.)

  • ShowHide

    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

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    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 1 - 10 µg/ml.(Exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence.)

Target

Relevance

HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

Cellular localization

Membrane

Alternative names

  • CA antibody
  • Capsid protein p24 antibody
  • Human immunodeficiency virus 1 antibody
  • Human immunodeficiency virus type 1 p24 antibody

Anti-HIV1 p24 antibody [39/5.4A] images:

  Western blot - HIV1 p24 antibody [39/5.4A] (ab9071)

Western blot - HIV1 p24 antibody [39/5.4A] (ab9071)

All lanes : Anti-HIV1 p24 antibody [39/5.4A] (ab9071) at 1/2000 dilution

Lane 1 : Mock transfected 293T cells - cell lysate
Lane 2 : 293T cells transfected with HIV DNA - cell lysate

Secondary
HRP conjugated goat anti-mouse IgG
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 24,25,41,55 kDa (why is the actual band size different from the predicted?)


Exposure time : 5 minutes

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-HIV1 p24 antibody [39/5.4A] (ab9071)

This product has been referenced in:

  • Joshi Aet al. Evidence of a role for soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) machinery in HIV-1 assembly and release. J Biol Chem 286:29861-71 (2011). WB.Read more (PubMed: 21680744) »
  • Lenassi Met al. HIV Nef is secreted in exosomes and triggers apoptosis in bystander CD4+ T cells. Traffic 11:110-22 (2010). WB.Read more (PubMed: 19912576) »

See all 3 publications for this product

Publishing research using ab9071? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"