HIV1 p24 antibody [39/5.4A] (ab9071)

Product Name 

HIV1 p24 antibody [39/5.4A]

Product type 

Primary antibodies

Description 

Mouse monoclonal [39/5.4A] to HIV1 p24

Specificity 

Human Immunodeficiency Virus Type 1(HIV-1) p24 protein.

Tested applications 
(see key)

ELISA, ICC/IF, RipA, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence. It also exhibits reactivity with viral lysates examined using Western blotting. In addition, the antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts. This antibody may be useful as a solid phase absorbent in an ELISA format.
For Western blotting and immunofluorescence we recommend 1-10 µg/mL of antibody.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.

Cellular localization 

Membrane

Research areas 

Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV

Relevance 

HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

The antibody is used to detect HIV-1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis.
Useful as solid phase for capture of p24 in an antigen ELISA format.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

39/5.4A

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from serum-free culture supernatant by ammonium sulfate precipitation

Form 

Liquid

Concentration 

Batch dependent within range: 0.98 - 1.00 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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