![Western blot - HIV1 p24 antibody [39/6.14] (ab9072) image](#ab9072_2.jpg)
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Products:Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
Overview
Product name
Anti-HIV1 p24 antibody [39/6.14]
See all HIV1 p24 products (28) ...
Description
Mouse monoclonal [39/6.14] to HIV1 p24
Specificity
Reacts with Human Immunodeficiency Virus Type 1(HIV 1) p24 protein. Cross reacts with Human Immunodeficiency Virus Type 2 and Simian Immunodeficiency Virus (SIV).
Tested applications
ICC/IF, WB, Radioimmunoprecipitation assay, ELISAmore details
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified from serum-free culture supernatant.
Primary antibody notes
The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. This antibody is useful as solid phase for capture of p24 in an antigen ELISA format
Clonality
Monoclonal
Clone number
39/6.14
Isotype
IgG1
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
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Western blot - HIV1 p24 antibody [39/6.14] (ab9072)
![Western blot - HIV1 p24 antibody [39/6.14] (ab9072) image](/ps/datasheet/Images/9/ab9072/ab9072_2.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
Radioimmunoprecipitation assay
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 1 - 10 µg/ml.
RipA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 10 µg/ml.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
Cellular localization
Membrane
Alternative names
- CA antibody
- Capsid protein p24 antibody
- Human immunodeficiency virus 1 antibody
- Human immunodeficiency virus type 1 p24 antibody
Anti-HIV1 p24 antibody [39/6.14] images:
Western blot - HIV1 p24 antibody [39/6.14] (ab9072)
All lanes : Anti-HIV1 p24 antibody [39/6.14] (ab9072) at 1/1000 dilution
Lane 1 : Untransfected 293T cells
Lane 2 : Human 293T cells transfected with HIV proviral DNA
Secondary
Goat anti-mouse HRP conjugated antibody
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 24,45 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
Ab9072 in Western blot.
This image is courtesy of an anonymous Abreview submitted on 13 February 2006.
References for Anti-HIV1 p24 antibody [39/6.14] (ab9072)
This product has been referenced in:
- Throm REet al. Efficient construction of producer cell lines for a SIN lentiviral vector for SCID-X1 gene therapy by concatemeric array transfection. Blood 113:5104-10 (2009). WB.Read more (PubMed: 19286997) »
- Gaskill PJet al. Human immunodeficiency virus (HIV) infection of human macrophages is increased by dopamine: a bridge between HIV-associated neurologic disorders and drug abuse. Am J Pathol 175:1148-59 (2009). ICC/IF.Read more (PubMed: 19661443) »
See all 3 publications for this product
Publishing research using ab9072? Please let us know so that we can cite the reference in this datasheet
![HIV1 p24 antibody [39/6.14] for WB in Human (9072)](/ps/Reviews/Images/ab4159_11905.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"