HIV1 p24 antibody [39/6.14] (ab9072)

Product Name 

HIV1 p24 antibody [39/6.14]

Product type 

Primary antibodies

Description 

Mouse monoclonal [39/6.14] to HIV1 p24

Specificity 

Human Immunodeficiency Virus Type 1(HIV-1) p24 protein.

Tested applications 
(see key)

ICC/IF, RipA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution (indirect IF).
RipA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 10 µg/ml (the antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane

Research areas 

Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV

Relevance 

HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

The antibody is used to detect HIV-1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis.
This antibody is useful as solid phase for capture of p24 in an antigen ELISA format

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

39/6.14

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from serum-free culture supernatant by ammonium sulfate precipitation

Form 

Liquid

Concentration 

Batch dependent within range: 2.01 - 2.01 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for HIV1 p24 antibody [39/6.14] images, references, products related to ab9072 and other tools.

HIV1 p24 antibody [39/6.14] images:

References for HIV1 p24 antibody [39/6.14] (ab9072)

  This product has been used in: (two most recent references)

Throm RE et al. Efficient construction of producer cell lines for a SIN lentiviral vector for SCID-X1 gene therapy by concatemeric array transfection. Blood 113:5104-10 (2009). WB. PubMed: 19286997

Gaskill PJ et al. Human immunodeficiency virus (HIV) infection of human macrophages is increased by dopamine: a bridge between HIV-associated neurologic disorders and drug abuse. Am J Pathol 175:1148-59 (2009). ICC/IF. PubMed: 19661443
If you publish research using ab9072 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding HIV1 p24 antibody [39/6.14]

Customer FAQs regarding HIV1 p24 antibody [39/6.14]

Protocols for HIV1 p24 antibody [39/6.14]

Price and availability of products related to HIV1 p24 antibody [39/6.14]

HIV1 p24 antibody [39/6.14] - related products: