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The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. Useful as solid phase for capture of p24 in an antigen ELISA format.
Our Abpromise guarantee covers the use of ab9071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Reactive with viral lysates . In addition, the antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts.|
|Radioimmunoprecipitation assay||Use at an assay dependent dilution. The antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts.|
|ELISA||Use at an assay dependent dilution.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml. Exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence.|
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [39/6.14] to HIV1 p24 (ab9072).|
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