Overview

  • Product name
    Anti-HIV1 p24 antibody (Biotin)
    See all HIV1 p24 primary antibodies
  • Description
    Goat polyclonal to HIV1 p24 (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Ab20774 specifically targets Human Immunodeficiency Virus Type 1 (HIV-1), p24.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
  • Immunogen

    Purified native p24 from strain IIIB

  • General notes

    The antibody consists of purified IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

Applications

Our Abpromise guarantee covers the use of ab20774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent concentration.

Target

  • Relevance
    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization
    Membrane
  • Alternative names
    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

References

This product has been referenced in:
  • Alonso-Camino V  et al. Lymphocyte display: a novel antibody selection platform based on T cell activation. PLoS One 4:e7174 (2009). ELISA . Read more (PubMed: 19777065) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lysed virus from 10 ul PBMC culture supernatant)
Loading amount
10 cells
Specification
Lysed virus from 10 ul PBMC culture supernatant
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Hoku West-Foyle

Verified customer

Submitted Nov 06 2009

Thank you for your enquiry. The number of biotin molecules per IgG molecule has not been determined. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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