HIV1 p24 protein (ab43037)

OK. Thank you very much! I will talk with my professor, then send you the Email.  

ANSWER:

You are welcome! Take all the time you need. I look forward to your reply whenever you are ready.

Thank you for your reply. it is very helpful.   The research I will carry out is to apply the magnetic detection echnique into HIV detection through sandwich assay.   From the information you provide, HIV1 p24 protein (ab73768) is the antigen I need, right? Since it can be detected by all three antibodies  Anti-HIV1 p24 antibody [ND1] (ab8246) and  Anti-HIV1 p24 antibody [ND1] (FITC) (ab81433) and Anti-HIV1 p24 antibody [39/5.4A] (ab9071).   I wonder if the ab81433 is the same as ab8246 except the ab81433 has FITC fluorogen which could be detected by fluoresence absorption. Right?      Looking for your reply. Thank you!    

ANSWER:

Thank you for your reply.

Unfortunately, I would not recommend the protein ab73768. This is only a fragment of the HIV1 p24 that lacks large parts of the protein such as the N-terminal amino acids (1-76). If the antibodies you would like to test recognise an epitope within these missing parts (which we do not know as they have not been mapped), these antibodies would not be suitable for the proposed assay. Therefore, I would recommend to use the protein ab43037 which is the full-length HIV1 p24 and will be detected by the antibodies.

Indeed, I can confirm that ab8246 and ab81433 are the same antibody clone, thus detecting the same epitope, and that ab81433 is conjugated to FITC which can be used in applications such as immunocytochemistry/immunofluorescence (ICC/IF). For your experiments it seems that you do not need the fluorochrome, so I would recommend ab8246. In summary, for the sELISA two antibodies that recognise different epitopes are required and the matching pair needs to be determined by the end user. To our knowledge, neither ab43037 nor ab8246/ab81433 nor ab9071 have yet been tested in sELISA. Therefore, I can offer a discount off a future purchase if you buy one or all of the products now, test it/them in sELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and which product you would like to test sELISA. I will then send a discount code. This code must be issued before purchasing the product(s) so please wait for my reply before ordering.

2. Purchase the product(s) either by phone, fax, or online (www.abcam.com).

3. Test it/them in sELISA.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody and protein ordered, respectively, and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product(s) turn out to be unsuitable for sELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

What is the difference between this four products?

ab43037 (HIV1 p24 protein)

ab73028 HIV1 p24 protein (His tag)

ab43036 HTLV I p24 protein

ab73768 HIV1 p24 protein

I wantt to buy one HIV antigen, and two HIV monoclonal antiboies for magnetic sandwich assay. Could you suggest one kit for me? (not elisa kit)

ANSWER:

Thank you for contacting us.

The differences are as follows:

ab43036 HTLV I p24 protein: This protein is the full length sequence of HTLV I p24, i.e. an in E. coli derived recombinant protein. The Human T-lymphotropic virus Type I (HTLV-1) is not identical with HIV. When Human immunodeficiency virus (HIV), the virus that causes AIDS, was characterized, it was originally named HTLV-III. Therefore, this protein seems not suitable for your experiments.

ab43037: This protein is the recombinant full length protein HIV1 p24, expressed in E.coli. This protein is guaranteed to work in Western blot and ELISA.

ab73028: This protein is the recombinant full length HIV1 p24 from HxB2 strain purified from Pichia pastoris. It contains a 6x histidine fusion partner which for example facilitates purification.

ab73768: This protein is only a recombinant fragment (amino acids 77-436) of the HIV1 p24 protein and is additionally fused to beta-galactosidase.

If you could provide some more information on the type of assay you would like to perform and let us know whether you are interested in HIV-1 or HIV-2, I would be happy to make a specific recommendation.

In case the assay is similar to a sandwich ELISA (sELISA), I can let you know that we have tested neither the proteins nor any antibodies for their suitability in sELISA, as this has to be tested for each pair individually. In this case, I would suggest to test ab43037 with the antibody clone [ND1] as in ab8246 or ab81433 and for example the monoclonal antibody ab9071. http://www.abcam.com/index.html?datasheet=8246 http://www.abcam.com/index.html?datasheet=8246. http://www.abcam.com/index.html?datasheet=81433 http://www.abcam.com/index.html?datasheet=81433. http://www.abcam.com/index.html?datasheet=9071 http://www.abcam.com/index.html?datasheet=9071.

If you decide to go ahead and purchase one of these products, please contact our Scientific Support team prior to the purchase as you might be eligible for our testing discount program.

Do not hesitate to contact us with more information to select the most suitable products for this experiment. I look forward to your reply.

I'm doing an ELISPOT-type assay with these products and am looking for a protocol.

ANSWER:

Thank you for your inquiry. I know that you said you're using nitrocellulose, and this protocol uses PVDF, but I hope you can adjust it accordingly.

http://www.abcam.com/index.html?pageconfig=resource&rid=11456 I hope this information helps. Please contact us with any other questions.