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Product Name
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HIV1 tat antibody [1]
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See all HIV1 tat antibodies (9)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [1] to HIV1 tat
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Immunogen
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Recombinant full length protein (Human)
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Specificity
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This antibody reacts with the rTat consensus sequences of HIV1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE).
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Epitope
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Mapped to amino acids 2-9 (EPVDPRLE - B subtype consensus).
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Tested applications
(see key)
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ELISA, IHC-P, RipA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/500 - 1/2000.
IHC-P: Use at an assay dependent dilution.
RipA: Use at an assay dependent dilution.
WB: 1/500 - 1/2000. Predicted molecular weight: 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
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Research areas
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Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
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Relevance
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HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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1
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody was purified by affinity chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Also known as clone 02-001.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HIV1 tat antibody [1] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for HIV1 tat antibody [1] (ab42359)
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This product has been used in: (two most recent references)
Henderson WW et al. Human immunodeficiency virus (HIV) type 1 Vpu induces the expression of CD40 in endothelial cells and regulates HIV-induced adhesion of B-lymphoma cells. J Virol 78:4408-20 (2004). PubMed: 15078922
Kjerrstrom A et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). PubMed: 11352667
See all 5 publication references for this product.
If you publish research using ab42359 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HIV1 tat
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HIV1 tat antibody [1] - more information
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Customer reviews (feedback) regarding HIV1 tat antibody [1] |
Customer FAQs regarding HIV1 tat antibody [1] |
Protocols for HIV1 tat antibody [1] |
Price and availability of products related to HIV1 tat antibody [1] |
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HIV1 tat antibody [1] - related products:
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HIV1 tat antibody [1] - other tools:
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Contact Abcam with a Technical Enquiry about HIV1 tat antibody [1] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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