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Products:Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
Overview
Product name
Anti-HIV1 tat antibody [1]
See all HIV1 tat products (7) ...
Description
Mouse monoclonal [1] to HIV1 tat
Specificity
This antibody reacts with the rTat consensus sequences of HIV1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE).
Tested applications
Radioimmunoprecipitation assay, IHC-P, IHC-Fr, ELISA, WBmore details
Immunogen
Recombinant full length protein (Human)
Epitope
Mapped to amino acids 2-9 (EPVDPRLE - B subtype consensus).
General notes
Also known as clone 02-001.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This antibody was purified by affinity chromatography.
Clonality
Monoclonal
Clone number
1
Isotype
IgG1
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RipA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 20651230)
ELISA
ELISA: 1/500 - 1/2000.ELISA: 1/500 - 1/2000.
WB
WB: 1/500 - 1/2000.WB: 1/500 - 1/2000.
Target
Relevance
HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
Cellular localization
Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
Alternative names
- 2interacting protein antibody
- 60 kDa Tat interactive protein antibody
- cPLA antibody
see all
References for Anti-HIV1 tat antibody [1] (ab42359)
This product has been referenced in:
- Henderson WWet al. Human immunodeficiency virus (HIV) type 1 Vpu induces the expression of CD40 in endothelial cells and regulates HIV-induced adhesion of B-lymphoma cells. J Virol 78:4408-20 (2004).Read more (PubMed: 15078922) »
- Kjerrström Aet al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001).Read more (PubMed: 11352667) »
See all 6 publications for this product
Publishing research using ab42359? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"