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This antibody isn't showing any signal in WB. |
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ANSWER: |
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. |
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AbID: 63957, Anti-HIV1 tat antibody [N3] Rating: Poor Image: Anti-HIV1 tat antibody [N3] for Western blot (HIV) Sample: Species: HIV Type: Recombinant protein Loading amount: 4 µg Specification: recombinant bacterial protein with TAT-domain and Treatment: IPTG Application: Application: Gel Running Conditions: Reduced Denaturing (12% SDS-PAGE) Blocking step: BSA as blocking agent for 1 hour 30 mins at 22°C Blocking Concentration: 5% Other detail: Dilution: 1/1000 Incubation time: 16 hours at 4°C Diluent: TBST + 5% BSA Secondary Antibody: Name: Non-Abcam Antibody was used: mouse-IgG-HRP Conjugation: HRP polymer Dilution: 1/5000 Detection: Detection method: ECL Exposure: 5 minutes and 0 seconds Bands: Specific: 37 kDa Non-specific: 80, 70, 30, 25 kDa Positive Control: specific detection with anti-His-Tag antibody Additional Data: |
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ANSWER: |
Thanks for testing this antibody. The text in your image describing the samples loaded to the gel have become quite small after reformatting for display on our website. If you could, please paste those notes into a reply to this email so I can upload them to the 'Additional notes' section of your review. I think this will be helpful for other users. |
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Phone call reporting problems with detecting tat-fusion protein with ab63957. |
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ANSWER: |
Thank you for contacting us and reporting the problems encountered with using Anti-HIV1 tat antibody [N3] (ab63957). |
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Koennen wir ein Testaliquot von ab63957 bekommen? |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
IHC-P image of HIV1 tat staining on Transgenic mouse olfactory bulb. Heat mediated antigen retrieval was performed using trisodium citrate (pH 6). The tissue section was permeabilization using Triton x100. To block non-specififc binding, sections were blocked in 2% BSA in PBS.
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
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