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Overview

  • Product nameHIV1 tat proteinSee all HIV1 tat proteins and peptides ...
  • Protein descriptionRecombinant full length HIV1 tat, 101 amino acids, MWt 11 kDa (Swiss-Prot O12161).
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Biological activityab83353 reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
  • FormLyophilised:The protein should be reconstituted in apirogenic sterile water or PBS buffer. The reconstituted solution has to be used immediately.
  • Storage instructionsStore at -70°C
  • Storage bufferPreservative: None
    Constituents: 0.1% Glycerol
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83353 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Blocking
    WB
    SDS-PAGE
  • Application notesBL: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 11 kDa.

    ab83353 has also been used in up-take, and rescue assays.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        p14Protein TatTransactivating regulatory protein
  • RelevanceHIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • Cellular localizationNuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.

    • References for HIV1 tat protein (ab83353)

    This product has been referenced in:
    • Qu J  et al. The Tat protein of human immunodeficiency virus-1 enhances hepatitis C virus replication through interferon gamma-inducible protein-10. BMC Immunol 13:15 (2012). Read more (PubMed: 22471703) »

    See 1 Publication for this product

    Publishing research using ab83353 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"