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Product Name
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HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate
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Product type
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Lysates
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Description
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HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate
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Tested applications
(see key)
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WB
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Application notes
(see key)
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Recommended dilutions
HL60 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane isrecommended for mini gel.
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Research areas
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Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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HL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies.
HL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
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Purity
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Whole Cell Lysate
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Storage buffer
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Whole lysate in 1 X SDS 5% b-mercaptoethanol
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Form
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It is supplied as whole cell lysate, in 1 x SDS sample buffer containing 5% b-mercaptoethanol.
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Cell line: HL60 (Human promyelocytic leukemia)
Growth media: RPMI & 10% FBS (Fetal bovine serum)
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate is on this datasheet. But please do contact us if you would like any reassurance! |
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References for HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate (ab7914)
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This product has been used in:
Manzel LJ et al. Regulation of Bacteria-Induced Intercellular Adhesion Molecule-1 by CCAAT/Enhancer Binding Proteins. Am J Respir Cell Mol Biol : (2008). PubMed: 18703796
If you publish research using ab7914 please let us know so that we can cite the reference on this datasheet.
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