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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate (ab7914)
Overview
Product name
HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate
General notes
Cell line: HL60 (Human promyelocytic leukemia)
Growth media: RPMI & 10% FBS (Fetal bovine serum)
Tested applications
Properties
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 6.80
Constituent: 5% Beta mercaptoethanol
Concentration
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Purity
Whole Cell Lysate
Lysate notes
HL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Research areas
Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Background
HL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (HL60 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.)
References for HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate (ab7914)
This product has been referenced in:
- Manzel LJet al. Regulation of Bacteria-Induced Intercellular Adhesion Molecule-1 by CCAAT/Enhancer Binding Proteins. Am J Respir Cell Mol Biol : (2008).Read more (PubMed: 18703796) »
See 1 publication for this product
Publishing research using ab7914? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"