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HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate (ab7914)

Q&A (2)Specific References (1)

Overview

  • Product nameHL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate
  • General notesCell line: HL60 (Human promyelocytic leukemia) Growth media: RPMI & 10% FBS (Fetal bovine serum)
  • Tested applicationsWB more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Lysate notes HL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research Areas
  • BackgroundHL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies.

    • Applications

    Our Abpromise guarantee covers the use of ab7914 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration.

    HL60 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

    References for HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate (ab7914)

    This product has been referenced in:
    • Manzel LJ  et al. Regulation of Bacteria-Induced Intercellular Adhesion Molecule-1 by CCAAT/Enhancer Binding Proteins. Am J Respir Cell Mol Biol : (2008). Read more (PubMed: 18703796) »

    See 1 Publication for this product

    Publishing research using ab7914 ? Please let us know so that we can cite the reference in this datasheet

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    Our customer would like to purchase positive control for ab102932.

    So I searched ab7914. Is it suitable for her purpose?

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    The product ab7914 can be used with ab102932.

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    この製品の細胞はどのぐらい培養したものでしょうか。

    いつもお世話になっております。

    先日お電話いただきましてありがとうございます。

    早速ですが、製造元より回答が届き��したので、お伝えいたします。

    HL60細胞を2-4週間（ログフェーズ成長）で培養してから、ライセートの製品化にしております。

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"