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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-HL60 antibody [IPO-M6]
Description
Mouse monoclonal [IPO-M6] to HL60
Specificity
Reacts with human leukemia cell line HL60 and immuno-precipitates with two proteins with MW of 48 and 52 kDa. ab24093 does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4.
Tested applications
IHC-Fr, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Tissue / cell preparation (Human): HL60 cell line.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
This antibody can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia were ab24093 positive. The antigen, defined with this mAb, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages were also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B-ALL were ab24093 negative.
Clonality
Monoclonal
Clone number
IPO-M6
Myeloma
P3x63-Ag8.653
Isotype
IgG1
Research areas
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
Target
Relevance
HL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies. The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia. The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro. Some reagents cause HL60 cells to differentiate to granulocyte-like cells, others to monocyte/macrophage-like cells. The HL60 cell genome contains an amplified c-myc proto-oncogene; c-myc mRNA levels are correspondingly high in undifferentiated cells but decline rapidly following induction of differentiation.
References for Anti-HL60 antibody [IPO-M6] (ab24093)
ab24093 has not yet been referenced specifically in any publications.
Publishing research using ab24093? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"