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Anti-HLA Class I antibody [W6/32] (FITC) (ab20313)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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Overview

Product name

Anti-HLA Class I antibody [W6/32] (FITC)
See all HLA Class I products (12) ...

Description

Mouse monoclonal [W6/32] to HLA Class I (FITC)

Conjugation

FITC

Specificity

This antibody recognizes a monomorphic determinant (alpha chain) of major histocompatibility HLA Class I antigens. We have data to indicate that this antibody may not cross react with Rabbit. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Tested applications

Flow Cyt, IHC-Frmore details

Cross reactivity

Reacts with

Cow, Cat, Human, Monkey

Predicted to work with

Chimpanzee, Gorilla

Immunogen

Purified human tonsil lymphocyte membranes.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Purified from tissue culture by chromatography.

Clonality

Monoclonal

Clone number

W6/32

Isotype

IgG2a

Research areas

Immunology >> Adaptive Immunity >> MHC >> Class I

  • Flow Cytometry - HLA Class I antibody [W6/32] (FITC) (ab20313)Flow Cytometry - HLA Class I antibody [W6/32] (FITC) (ab20313) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab20313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

HLA CLass I is involved in the presentation of foreign antigens to the immune system.

Cellular localization

Plasma membrane

Alternative names

  • Antigen Presenting Molecule antibody
  • Cw*1701 antibody
  • D6S204 antibody
  • FLJ27082 antibody
  • HLA A antibody
  • HLA B antibody
  • HLA C antibody
  • HLA class I histocompatibility antigen A 1 alpha chain antibody
  • HLA Class I Histocompatibility Antigen A Alpha Chain antibody
  • HLA class I histocompatibility antigen B 7 alpha chain antibody
  • HLA Class I Histocompatibility Antigen B Alpha Chain antibody
  • HLA Class I Histocompatibility Antigen C Alpha Chain antibody
  • HLA class I histocompatibility antigen Cw 1 alpha chain antibody
  • HLA JY3 antibody
  • HLAA antibody
  • HLAB antibody
  • HLAC antibody
  • HLC C antibody
  • Human Leucocyte Antigen C antibody
  • Major Histocompatibility Complex Class I A antibody
  • Major Histocompatibility Complex Class I B antibody
  • Major Histocompatibility Complex Class I C antibody
  • MHC class I antigen A*1 antibody
  • MHC Class I Antigen antibody
  • MHC class I antigen B*7 antibody
  • MHC class I antigen Cw*1 antibody
  • PSORS1 antibody
see all

Database links

see all

Anti-HLA Class I antibody [W6/32] (FITC) images:

  Flow Cytometry - HLA Class I antibody [W6/32] (FITC) (ab20313)

Flow Cytometry - HLA Class I antibody [W6/32] (FITC) (ab20313)

Overlay histogram showing Jurkat cells stained with ab20313 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20313, 1/20 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) with secondary antibody DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in Jurkat cells fixed with methanol (5 min) used under the same conditions.

Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-HLA Class I antibody [W6/32] (FITC) (ab20313)

This product has been referenced in:

  • Kalkunte SS  et al. Vascular endothelial growth factor C facilitates immune tolerance and endovascular activity of human uterine NK cells at the maternal-fetal interface. J Immunol 182:4085-92 (2009). Flow Cyt; Human.Read more (PubMed: 19299706) »
  • Szatmari I  et al. PPARgamma controls CD1d expression by turning on retinoic acid synthesis in developing human dendritic cells. J Exp Med 203:2351-62 (2006).Read more (PubMed: 16982809) »

See all 13 publications for this product

Publishing research using ab20313? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"