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Product Name 

HLA Class 1 A10, A11 antibody [4i90]

Product type 

Primary antibodies

Description 

Mouse monoclonal [4i90] to HLA Class 1 A10, A11

Immunogen 

HLA Class 1 Antigen A10, A11 (Human).

Reacts with 
(species key)

Hu

Specificity 

This antibody reacts to HLA Class 1 Antigen A10, A11. Additional crossreactivity occurs with related proteins. Specificity was determined at a 1/10 dilution by the microcytotoxicity test under standard conditions.

Tested applications 
(see key)

Flow Cyt, ICC/IF


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.

Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100 µl of 10X working dilution.
Expected results: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.

This antibody is also suitable for use in Cell Typing and Chimerism Studies.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Immunology >> Adaptive Immunity >> MHC >> Class I

Relevance 

Human leukocyte antigen (HLA) Class 1 is involved in the presentation of foreign antigens to the immune system.

 

Alternative names HLA Class 1 A10, A11 antibody [4i90] (ab33804)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3105    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

4i90

Isotype 

IgM

Purity 

Ascites

Storage buffer 

Preservative: None
Constituents: Ascites

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HLA Class 1 A10, A11 antibody [4i90] is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to HLA Class 1 A10, A11

 

HLA Class 1 A10, A11 antibody [4i90] - more information

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HLA Class 1 A10, A11 antibody [4i90] - other tools:

Contact Abcam with a Technical Enquiry about HLA Class 1 A10, A11 antibody [4i90]

 

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