Anti-HLA Class I antibody [W6/32] - Azide free (ab23755)

Testing discount code for ab23755 to try in mouse.

ANSWER:

DISCOUNT CODE: *********
Expiration date: *********

I am very pleased to hear you would like to accept our offer and test ab23755 in mouse tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for your email. I think I may have not explained myself correctly – I’m actually looking for a positive control (eg overexpression cell lysate or purified protein) that I could use to confirm the antibody against HLA Class I is working correctly. I have a (weak) band of the expected size on my WB using human PBMCs but I would like to also have that positive control for completeness if possible. Thanks for your help and best wishes,

ANSWER:

Thank you for your response. Lysates from Jurkat or HeLa cells or from human tonsil tissue can be used as positive control. Currently, we have do not have HLA Class I over-expression cell lysate in our catalogue. It may be worth optimizing the working concentration/dilution of this antibody to get stronger band on the blot. I hope this helps and if I can assist further, please do not hesitate to contact me.

To whom it may concern, Do you have any loading controls that would be suitable to use in a Western blot with any of your anti-HLA class 1 antibodies such as ab23755? Thanks and best wishes,

ANSWER:

Thank you for your enquiry and your interest in our products. Anti-alpha tubulin (clone TU-01) can be used as loading control together with antibody HLA Class I (ab23755) . Currently, we have several forms of this antibody ie. unconjugated as well as conjugated: - ab7750 - Anti-alpha Tubulin antibody [TU-01] - ab18247 - Anti-alpha Tubulin antibody [TU-01] (FITC) - ab46708 - Anti-alpha Tubulin antibody [TU-01] (Dyomics® 547) - ab74696 - Anti-alpha Tubulin antibody [TU-01] (Biotin) - ab78108 - Anti-alpha Tubulin antibody [TU-01] I hope this helps and if I can assist further, please do not hesitate to contact me.

I would like to test this antibody in rat paraffin-embedded tissue sections, IHC-P, an untested application.

ANSWER:

I am very pleased to hear you would like to accept our offer and test ab23755 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thanks for the reply. I still want to make sure of one thing. Are you sure this is the right antibody you sent to us? In your own test of product, does the antibody recognize multiple bands around 50KD as well? It will be great if you could send me a photo of your test western blot for this antibody.

ANSWER:

Thank you for your patience in awaiting a response. I have been in contact with the source of this product in order to source further information. A431 human skin squamos cell carcinoma cell line appears to have been used to test this product; however unfortunately I have been unable to source a full blot showing below the predicted KDa so it is hard for me to comment on additional banding that may be present. I apologise for not being able to source any further information int his instance.