Recombinant Anti-HLA Class I antibody [W6/32] (ab22432)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [W6/32] to HLA Class I
- Suitable for: Flow Cyt, ICC/IF, IHC-Fr
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HLA Class I antibody [W6/32]
See all HLA Class I primary antibodies -
Description
Mouse monoclonal [W6/32] to HLA Class I -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human HLA Class I. Purified human tonsil lymphocyte membranes.
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Positive control
- IHC-Fr: Human heart tissue. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 25th March 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
W6/32 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 0.2 µg/ml.
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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IHC-Fr |
Use a concentration of 0.05 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 0.2 µg/ml. |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-Fr
Use a concentration of 0.05 µg/ml. |
Target
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Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P04439 Human
- SwissProt: P13747 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Images
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Immunohistochemical analysis of ab22432 10% paraformaldehyde fixed endothelial cells in frozen Human spleen tissue Human heart tissue labeling HLA Class I with ab22432 at 0.05µg/ml. Detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% PBS-Tween permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling HLA Class I with ab22432 at 1µg/mL, blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab92494 at 1µg/mL and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% PBS-Tween permeabilized negative cell line K562 labelling HLA Class I with ab22432 at 1µg/mL, blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab227805 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Flow cytometry overlay histogram showing left Jurkat positive cells and right negative K562 cells stained with ab22432 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab22432) (1x106 in 100 μl at 0.2 μg/ml) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at for 30 min on ice. Isotype control antibody (black line) was mouse IgG2aκ (ab18413) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab22432 has been referenced in 32 publications.
- Kurdi M et al. The Assessment of Major Histocompatibility Complex (MHC) Class-I Expression in Different Neuromuscular Diseases. Degener Neurol Neuromuscul Dis 11:61-68 (2021). PubMed: 35002356
- Choi J et al. Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutations. Cell Rep Methods 1:100084 (2021). PubMed: 35474673
- Mahameed M et al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nat Commun 11:1304 (2020). PubMed: 32161259
- Grandon B et al. HLA-B27 alters BMP/TGFß signalling in Drosophila, revealing putative pathogenic mechanism for spondyloarthritis. Ann Rheum Dis 78:1653-1662 (2019). PubMed: 31563893
- Mikkelsen K et al. Carcinoembryonic Antigen (CEA)-Specific 4-1BB-Costimulation Induced by CEA-Targeted 4-1BB-Agonistic Trimerbodies. Front Immunol 10:1791 (2019). PubMed: 31417564