Overview

  • Product nameAnti-HLA Class II DRB1 antibodySee all HLA Class II DRB1 primary antibodies ...
  • Description
    Rabbit polyclonal to HLA Class II DRB1
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Cat, Dog, Monkey
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 56 and 260 of Human HLA Class II DRB1 (AAH07920).

  • Positive control
    • A431, H1299, HeLa, MOLT4, Raji cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab98108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.

Target

  • FunctionBinds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Sequence similaritiesBelongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to sorting into the endosome system and down-regulation of MHC class II.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2B1F_HUMAN antibody
    • DR1 antibody
    • DR16 antibody
    • DR5 antibody
    • DR8 antibody
    • DRB1 antibody
    • DRw10 antibody
    • DW2.2/DR2.2 antibody
    • FLJ75017 antibody
    • FLJ76359 antibody
    • HLA class II antigen beta chain antibody
    • HLA class II histocompatibility antigen, DR-1 beta chain antibody
    • HLA class II histocompatibility antigen, DRB1-15 beta chain antibody
    • HLA-DR-beta 1 antibody
    • HLA-DR1B antibody
    • HLA-DRB antibody
    • HLA-DRB1 antibody
    • human leucocyte antigen DRB1 antibody
    • Leucocyte antigen DR beta 1 chain antibody
    • leucocyte antigen DRB1 antibody
    • lymphocyte antigen DRB1 antibody
    • major histocompatibility complex, class II, DR beta 1 antibody
    • MHC class II antigen antibody
    • MHC class II antigen DRB1*15 antibody
    • MHC class II antigen HLA-DR13 antibody
    • MHC class II HLA-DR beta 1 chain antibody
    • MHC class II HLA-DR-beta cell surface glycoprotein antibody
    • MHC class II HLA-DRw10-beta antibody
    • SS1 antibody
    see all

Anti-HLA Class II DRB1 antibody images

  • Anti-HLA Class II DRB1 antibody (ab98108) at 1/1000 dilution + H1299 whole cell lyaste at 30 µg

    Predicted band size : 30 kDa

References for Anti-HLA Class II DRB1 antibody (ab98108)

ab98108 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"