Anti-HLA Class II DRB1 antibody (ab98108)

Overview

• Product nameAnti-HLA Class II DRB1 antibodySee all HLA Class II DRB1 primary antibodies ...
• Description
  Rabbit polyclonal to HLA Class II DRB1
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Cow, Cat, Dog, Monkey
• Immunogen

  Recombinant protein fragment corresponding to a region within amino acids 56 and 260 of Human HLA Class II DRB1 (AAH07920).

• Positive controlA431, H1299, HeLa, MOLT4, Raji cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Immunology
  • Adaptive Immunity
  • MHC
  • Class II

Applications

Target

• FunctionBinds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
• Sequence similaritiesBelongs to the MHC class II family.
  Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
• Post-translational
  modificationsUbiquitinated by MARCH1 and MARCH8 at Lys-254 leading to sorting into the endosome system and down-regulation of MHC class II.
• Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

Target information above from: UniProt accession P01911 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3123 Human
  • Entrez Gene: 14969 Mouse
  • Omim: 142857 Human
  • SwissProt: P01911 Human
  • SwissProt: P18468 Mouse
  • Unigene: 534322 Human
  • Unigene: 696211 Human
  • Unigene: 22564 Mouse

• Alternative names
  2B1F_HUMAN antibodyDR1 antibodyDR16 antibody

  DR5 antibodyDR8 antibodyDRB1 antibodyDRw10 antibodyDW2.2/DR2.2 antibodyFLJ75017 antibodyFLJ76359 antibodyHLA class II antigen beta chain antibodyHLA class II histocompatibility antigen, DR-1 beta chain antibodyHLA class II histocompatibility antigen, DRB1-15 beta chain antibodyHLA-DR-beta 1 antibodyHLA-DR1B antibodyHLA-DRB antibodyHLA-DRB1 antibodyhuman leucocyte antigen DRB1 antibodyLeucocyte antigen DR beta 1 chain antibodyleucocyte antigen DRB1 antibodylymphocyte antigen DRB1 antibodymajor histocompatibility complex, class II, DR beta 1 antibodyMHC class II antigen antibodyMHC class II antigen DRB1*15 antibodyMHC class II antigen HLA-DR13 antibodyMHC class II HLA-DR beta 1 chain antibodyMHC class II HLA-DR-beta cell surface glycoprotein antibodyMHC class II HLA-DRw10-beta antibodySS1 antibody

  see all

Anti-HLA Class II DRB1 antibody images

• 

  Western blot - HLA Class II DRB1 antibody (ab98108)
  Anti-HLA Class II DRB1 antibody (ab98108) at 1/1000 dilution + H1299 whole cell lyaste at 30 µg
  
  Predicted band size : 30 kDa