Recombinant Anti-HLA DMB antibody [EPR7982] (ab131273)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7982] to HLA DMB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HLA DMB antibody [EPR7982]
See all HLA DMB primary antibodies -
Description
Rabbit monoclonal [EPR7982] to HLA DMB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colon tissue and tonsil tissue. ICC/IF: Raji cells and 293T cells. WB: HEK-293T cells transfected with a human HLA-DMB expression vector containing a his tag whole cell lysate, Raji, and Daudi cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7982 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Relevance
HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. -
Cellular localization
Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=Localizes to late endocytic compartment. Associates with lysosome membranes. -
Database links
- Entrez Gene: 3109 Human
- Omim: 142856 Human
- SwissProt: P28068 Human
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Alternative names
- class II antigen HLA-DM beta chain antibody
- class II histocompatibility antigen antibody
- Class II histocompatibility antigen M beta chain antibody
see all
Images
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All lanes : Anti-HLA DMB antibody [EPR7982] (ab131273) at 1/1000 dilution
Lane 1 : HEK-293T cells transfected with a human HLA-DMB expression vector containing a his tag, whole cell lysate
Lane 2 : HEK-293T cells transfected with a human NEK6 expression vector containing a his tag, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 26 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBSTThis antibody does not cross-react with human NEK6.
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All lanes : Anti-HLA DMB antibody [EPR7982] (ab131273) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Daudi cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HLA DMB with ab131273 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling HLA DMB with ab131273 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling HLA DMB with primary antibody anti-HLA DMB (ab131273) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution. Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged HLA-DMB expression vector, no staining in 293T cells transfected with myc-tagged NEK6 expression vector. Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) (ab202008) was used as counterstain at 1/100 dilution. The nuclear counter stain is DAPI (blue).
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Immunofluorescence analysis of Raji cells labelling HLA DMB with ab131273 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab131273 has been referenced in 3 publications.
- Baleeiro RB et al. MHC class II molecules on pancreatic cancer cells indicate a potential for neo-antigen-based immunotherapy. Oncoimmunology 11:2080329 (2022). PubMed: 35655709
- Kanangat S et al. Circulating histocompatibility antigen (HLA) gene products may help differentiate benign from malignant indeterminate pulmonary lesions. Hum Immunol 79:558-563 (2018). WB . PubMed: 29656111
- Wang J et al. HLA-DMB restricts human T-cell leukemia virus type-1 (HTLV-1) protein expression via regulation of ATG7 acetylation. Sci Rep 7:14416 (2017). PubMed: 29089548