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Anti-HLA E antibody [MEM-E/02] (ab2216)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab2216 for help.

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2 questions for ab2216

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Question 1

Monday 08-November-2004

I am looking for anti-HLA-E antibodies for FACS and Western. I found 4 anti-HLA-E antibodies from your website and I have some technical questions about them, such as, what does "cross-reacts with human" mean on your data sheet when it states "it does not cross-react with HLA-A,-B, -C or -G", and for the other three anti-HLA-E antibodies how serious the cross-reactive problem is. Do you have a technical service number for customers in the US? For ordering, do you have special discount for a bulk purchasing?

ANSWER:

  

Thank you for your email. Cross-reactivity on our product datasheets refers to what species the antibody has been tested for cross-reactivity with. For example, in the case of ab2216, the antibody has been shown to detect human HLA E. It has not yet been determined as to whether the antibody will detect mouse HLA E, for instance. All the information, including cross-reactivity, that we have regarding our antibodies is located on the online datasheets. We do have discounts for bulk orders - ordering more than 2 vials of the same antibody. If you have any additional questions, please do let me know.

Question 2

Monday 25-November-2002

I have used the HLA-E antibody for Western blotting and gotten two seemingly very specific bands on my film. What are the molecular weight of the HLA-E part(s) that the antibody regognises? Many thanks. Best Regards

ANSWER:

  

This antibody recognizes the 43 kDa HLA-E heavy chain and does not cross-react with denatured form of HLA-A,-B,-C alleles (no band at 45 kDa observed). The specifity was confirmed at HLA-G/HLA-E. Workshop and publication should appear soon in Human Immunology.

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